지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Dianthus caryophyllus (carnation) is a globally important ornamental plant. Tissue culture techniques have been used for the commercial production of carnation; however, the micropropagation of carnation has been impeded due to the occurrence of hyperhydricity during the shoot multiplication process or micropropagation stage 2. In the present study, the effects of different concentrations of rare earth elements on the reduction of hyperhydricity in micropropagated carnation were investigated. Nodal explants of D. caryophyllus ‘13827’ were cultured on Murashige and Skoog medium containing 1.0 mg·L-1 6-benzyladenine and 0.5 mg·L-1 indole-3-acetic acid with 3.0% (w/v) sucrose and 0.8% (w/v) agar (control medium). The medium was supplemented with lanthanum nitrate, La(NO3)3, cerium nitrate, Ce(NO3)3, or neodymium chloride, NdCl3 at a concentration of 0.05, 0.1, or 0.15 mM. Hyperhydricity was observed in 68.9% of the cultures produced on the control medium. The lowest percentage (42.2%) of hyperhydricity was observed in plants propagated on the medium supplemented with 0.05 mM Ce(NO3)3. Furthermore, the soluble protein concentration was higher in both the non-hyperhydric and hyperhydric plants produced on the medium supplemented with 0.05 mM Ce(NO3)3, whereas the activity of catalase, guaiacol peroxidase, and ascorbate peroxidase was lower in comparison with the media lacking Ce3+. The results of this study suggest that supplementation of the culture medium with 0.05 mM Ce(NO3)3 alleviates oxidative stress and reduces hyperhydricity in carnation during the adventitious shoot multiplication stage.
목차
등록된 정보가 없습니다.
참고문헌 (41)
참고문헌 신청
Ali A / 2008 / An efficient protocol for in vitro propagation of carnation (Dianthus caryophyllus) / Pak J Bot 40:111~121
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding / Anal Biochem 72:248~254
Cakmak I / 1992 / Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean Leaves / Plant Physiol 98:1222~1227
Cassells AC / 2001 / Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers / Plant Cell Tissue Organ Cult 64:145~157
Chen SA / 2004 / Promotion of the growth of Crocus sativus cells and the production of crocin by rare earth elements / Biotechnol Lett 26:27~30
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding / Anal Biochem 72:248~254
Cakmak I / 1992 / Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean Leaves / Plant Physiol 98:1222~1227
Cassells AC / 2001 / Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers / Plant Cell Tissue Organ Cult 64:145~157
Chen SA / 2004 / Promotion of the growth of Crocus sativus cells and the production of crocin by rare earth elements / Biotechnol Lett 26:27~30
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Potential Reduction of Percent Hyperhydricity by Supplementation of Rare Earth Elements during Adventitious Shoot Multiplication of Carnation In Vitro
한국원예학회 학술발표요지
2017 .05
-
Reduction of Hyperhydricity by the Supplementation of Rare Earth Elements During Micropropagation of Carnation
한국원예학회 학술발표요지
2018 .10
-
Physiological and Molecular Insights on the Silicon-Mediated Recovery of Hyperhydricity in Carnation
한국원예학회 학술발표요지
2017 .05
-
Effect of Light Intensity, CO₂ Enrichment, and Silicon Supplementation on Recovery from Hyperhydricity of Carnation Plantlets ‘Inca Magic’
한국원예학회 학술발표요지
2017 .10
-
Exogenous Supplementation of Sodium Hydrosulfide Suppresses Incidence of Hyperhydricity and Promotes Growth and Development of Micropropagated Carnation
한국원예학회 학술발표요지
2018 .05
-
Red and Blue Light Emitting Diodes (LEDs) Mitigate Hyperhydricity in In Vitro-Grown Carnation (Dianthus caryophyllus)
한국원예학회 학술발표요지
2016 .05
-
Effect of Immersion Duration on Shoot Growth and Development of Plantlets of Carnation ‘Dreambyul’ In Vitro
한국원예학회 학술발표요지
2018 .10
-
편백의 다신초 유도 및 발근을 통한 식물체 재분화
Journal of Plant Biotechnology
2019 .01
-
Adventitious root culture for secondary metabolite production in medicinal plants: A Review
Journal of Plant Biotechnology
2019 .01
-
Establishment of protocol for genetic transformation of carnation with 1-aminocyclopropane-carboxylate deaminase (acdS) gene
Journal of Plant Biotechnology
2021 .01
-
Effect of Plant Growth Regulators on the Axillary Shoot Multiplication of Tree Peonies In Vitro
한국원예학회 학술발표요지
2016 .10
-
Effect of Immersion Frequency on Shoot Growth and Development of Plantlets of Carnation ‘Dreambyul’ in Temporary Immersion System
한국원예학회 학술발표요지
2018 .05
-
작약(Paeonia lactiflora Pall.) 뿌리절편 유래 캘러스 배양으로부터 부정근발생을 위한 최적 배양조건
Journal of Plant Biotechnology
2022 .09
-
Rapid and Effective Multiplication of Garlic using Shoots and Seed Bulbs Derived from Meristem Culture
한국원예학회 학술발표요지
2019 .10
-
Virus Elimination From Adventitious Shoot Regeneration in Malus pumila cv. Hongro
한국원예학회 학술발표요지
2017 .05
-
Multiple shoot induction and plant regeneration from axillary buds of Magnolia ‘Vulcan’
Journal of Plant Biotechnology
2020 .01
-
Ventilation and Temperature Treatment Improve Recovery from Hyperhydricity in Dianthus caryophyllus by Modulating Physiological and Molecular Mechanisms
한국원예학회 학술발표요지
2016 .10
-
Plant regeneration via callus-mediated organogenesis in commercial variety of Chuanbeichai No. 1 in Bupleurum chinense DC
Plant Biotechnology Reports
2023 .04
-
Effect of Difference in Day and Night Temperature during In Vitro Propagation of Carnation ‘Green Beauty’ and ‘Purple Beauty’
한국원예학회 학술발표요지
2016 .10
-
Adventitious root cultures of Clitoria ternatea L. and its potential as a memory enhancer alternative
Plant Biotechnology Reports
2021 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0