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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 101 - 109 (9page)
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In this study, we identified and evaluated the genetic relationships among Cinnamomum plants, which are used in traditional medicine. We also attempted to monitor the distribution of traditional medicines derived from Cinnamomum cassia by using DNA barcoding and a species-specific DNA marker. Plants of the genus Cinnamomum, and in particular C. cassia, are commonly used as medicinal herbs in the form of Cinnamomi Ramulus, Cinnamomi Cortex, and Cassiae Cortex Interior. However, it is difficult to distinguish among different Cinnamomum species based on morphological features, and so to overcome this limitation, nucleotide sequences of the internal transcribed spacer (ITS) region of Cinnamomum DNA were determined and compared. On the basis of the discrepancy in determined ITS sequences, a 408-bp product, amplified by the primer pair CC F1/CC R3, was developed as a C. cassia-specific DNA marker. Using the developed DNA marker in combination with the ITS 2 nucleotide sequence, we monitored imported and commercially supplied medicinal products derived from Cinnamomum plants in markets in Korean, China, and Japan. The results revealed that most of the specimens monitored were derived from C. cassia.
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참고문헌 (20)
참고문헌 신청
Abeysinghe PD / 2009 / Molecular characterization of Cinnamon (Cinnamomum verum Presl) accessions and evaluation of genetic relatedness of Cinnamon species in Sri Lanka based on trnL intron region, intergenic spacers between trnT-trnL, trnL-trnF, trnH-psbA and nuclear ITS / Res J Agric Biol Sci 5:1079~1088
Chen SL / 2009 / Strategy and key technique of identification of Chinese herbal medicine using DNA barcoding / Chin J Nat Med 7:322~327
Chen S / 2010 / Validation of the ITS2 region as a novel DAN barcode for identifying medicinal plants species / PLoS ONE 5:e8613
Chiou SJ / 2007 / Authentication of medicinal herbs using PCR-amplified ITS2 with specific primers / Planta Med 73:1421~1426
Ho KY / 2015 / A comparison of nrDNA internal transcribed spacer (ITS) loci for phylogenetic inference and authentication among Cinnamomum osmophloeum and related species in Taiwan / Afr J Biotech 14:1088~1096
Chen SL / 2009 / Strategy and key technique of identification of Chinese herbal medicine using DNA barcoding / Chin J Nat Med 7:322~327
Chen S / 2010 / Validation of the ITS2 region as a novel DAN barcode for identifying medicinal plants species / PLoS ONE 5:e8613
Chiou SJ / 2007 / Authentication of medicinal herbs using PCR-amplified ITS2 with specific primers / Planta Med 73:1421~1426
Ho KY / 2015 / A comparison of nrDNA internal transcribed spacer (ITS) loci for phylogenetic inference and authentication among Cinnamomum osmophloeum and related species in Taiwan / Afr J Biotech 14:1088~1096
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