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논문 기본 정보

자료유형
학술저널
저자정보
천경성 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm) 백정호 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm) 조영일 (Seed Industry Promotion Center Foundation of Agri. Tech. Commercialization & Transfer (FACT) Gi) 정영민 (Seed Industry Promotion Center Foundation of Agri. Tech. Commercialization & Transfer (FACT) Gi) 이윤영 (Seed Industry Promotion Center Foundation of Agri. Tech. Commercialization & Transfer (FACT) Gi) 오준 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm) 원용재 (Department of Central Area Crop Science National Institute of Crop Science Rural Development Admini) 강도유 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm) 오효자 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm) 김송림 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm) 최인찬 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm) 윤인선 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm) 김경환 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm) 한정헌 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm) 지현소 (Department of Agricultural Biotechnology National Institute of Agricultural Sciences Rural Developm)
저널정보
한국육종학회 Plant Breeding and Biotechnology Plant Breeding and Biotechnology Vol.6 No.4
발행연도
2018.1
수록면
391 - 403 (13page)

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Genome resequencing by next-generation sequencing technology can reveal numerous single nucleotide polymorphisms (SNPs) within a closely-related cultivar group, which would enable the development of sufficient SNP markers for mapping and the identification of useful genes present in the cultivar group. We analyzed genome sequence data from 13 Korean japonica rice varieties and discovered 740,566 SNPs. The SNPs were distributed at 100-kbp intervals throughout the rice genome, although the SNP density was uneven among the chromosomes. Of the 740,566 SNPs, 1,014 SNP sites were selected on the basis of polymorphism information content (PIC) value higher than 0.4 per 200-kbp interval, and 506 of these SNPs were converted to Kompetitive Allele-Specific PCR (KASP) markers. The 506 KASP markers were tested for genotyping with the 13 sequenced Korean japonica rice varieties, and polymorphisms were detected in 400 KASP markers (79.1%) which would be suitable for genetic analysis and molecular breeding. Additionally, a genetic map comprising 205 KASP markers was successfully constructed with 188 F2 progenies derived from a cross between the varieties, Junam and Nampyeong. In a phylogenetic analysis with 81 KASP markers, 13 Korean japonica varieties showed close genetic relationships and were divided into three groups. More KASP markers are being developed and these markers will be utilized in gene mapping, quantitative trait locus (QTL) analysis, marker-assisted selection and other strategies relevant to crop improvement.

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참고문헌 (30)

참고문헌 신청
Anderson JA / 1993 / Optimizing parental selection for genetic linkage maps / Genome 36:181~186 google schola Arai-Kichise Y / 2014 / Genome-wide DNA polymorphisms in seven rice cultivars of temperate and tropical japonica groups / PLoS One 9:e86312 google schola Bernardo R. / 2008 / Molecular markers and selection for complex traits in plants:Learning from the last 20 years / Crop Sci 48:1649~1664 google schola Ertiro BT / 2015 / Comparison of Kompetitive Allele Specific PCR(KASP)and genotyping by sequencing(GBS)for quality control analysis in maize / BMC Genomics 16:908 google schola Gao LZ / 2008 / Nonindependent domestication of the two rice subspecies, Oryza sativa ssp. indica and ssp. japonica, demonstrated by multilocus microsatellites / Genetics 179:965~976 google schola

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