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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 61 - 70 (10page)
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Glutathione S-transferase (GST) genes from transcripts of Vitis flexuosa leaves infected with Elsinoe ampelina were characterized and analyzed for their expression using primers based on specific regions. Comparison of deduced amino acid sequences from GST transcripts of V. flexuosa showed that the score of the deduced amino acid identity ranged from 43.38% (VfGST26625 and VfGST774) to 6.67% (VfGST13892 and VfGST774). Primary and secondary structure analysis was performed using the ProtParam and Self-Optimized Prediction Method with Alignment software. A phylogenetic tree was constructed from the GST proteins by the neighbor joining method using MEGA 6.0 to investigate the relationship among VfGST, VvGST, and AtGST proteins. To evaluate the differential expression pattern of GST genes by real-time polymerase chain reaction (PCR), primers specific to unique regions in each gene were obtained by alignment of the sequences. Real-time PCR revealed that GST genes were expressed differentially in the leaves of V. flexuosa infected with Botrytis cinerea, E. ampelina, and Rhizobium vitis. The expression of VfGST26625 was up-regulated, while that of others were down-regulated among five GSTs in all grapevine leaves inoculated with each pathogen. The results provided herein improve our understanding of defense responses to various pathogen attacks in grapevines.
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참고문헌 (38)
참고문헌 신청
Adam A / 1989 / Consequence of O2-generation during a bacterially induced hypersensitive reaction in tobacco: deterioration of membrane lipids / Physiol. Mol. Plant Pathol 34:13~26
Ahn SY / 2014 / De novo transcriptome assembly of Vitis flexuosa grapevines inoculated with Elsinoe ampelina / Plant Genet. Resour 12:S130~S133
Alvarez ME / 1998 / Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity / Cell 92:773~784
Bézier A / 2002 / Study of defenserelated gene expression in grapevine leaves and berries infected with Botrytis cinerea / Eur. J. Plant Pathol 108:111~120
Buxbaum E / 2007 / Fundamentals of protein structure and function / Springer Science Business Media
Ahn SY / 2014 / De novo transcriptome assembly of Vitis flexuosa grapevines inoculated with Elsinoe ampelina / Plant Genet. Resour 12:S130~S133
Alvarez ME / 1998 / Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity / Cell 92:773~784
Bézier A / 2002 / Study of defenserelated gene expression in grapevine leaves and berries infected with Botrytis cinerea / Eur. J. Plant Pathol 108:111~120
Buxbaum E / 2007 / Fundamentals of protein structure and function / Springer Science Business Media
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