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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한임상검사정도관리협회 Journal of Laboratory Medicine And Quality Assurance Journal of Laboratory Medicine And Quality Assurance Vol.41 No.4
- 발행연도
- 2019.1
- 수록면
- 214 - 219 (6page)
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초록· 키워드
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Background: Cell-free DNA (cfDNA) provides unique potential as a biomarker for cancer patients or in the field of prenatal care. Following extraction from plasma, cfDNA should be quantified before downstream analysis. The standardization of the quantification methods is essential for application of these techniques to laboratory practice. We aimed to compare three different quantification methods (spectrophotometry, fluorometry, and the electrophoresis-based method) and to determine the contributor(s) to the differences, if any. Methods: A total of 135 plasma samples obtained from cancer patients (n=71) and normal individuals (n=15) and cfDNA were extracted from the samples. The extracted cfDNA was quantified using three different methods: NanoDrop spectrophotometry, Quantus fluorometry, and the electrophoresis-based 4200 TapeStation method. Results: NanoDrop exhibited the highest estimates (median, 12.3 ng/uL). The estimates of Quantus (median, 0.33 ng/uL) were higher than those of 4200 TapeStation (median, 0.22 ng/ uL). Quantus and TapeStation were significantly correlated (r =0.802, P<0.001). The Bland- Altman plot showed a positive bias of 0.24 ng/uL for Quantus compared with TapeStation. The difference between Quantus and TapeStation values demonstrated a significantly moderate correlation with the amount of high molecular weight DNA (r =0.357, P<0.001). Conclusions: Nanodrop is considered as a non-specific method of cfDNA quantification. Quantus depicted more specific quantification compared to Nanodrop; however, TapeStation may be used in parallel or as a complementary method as it enables to determine the size distribution of DNA fragments.
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참고문헌 (25)
참고문헌 신청
Wan JC / 2017 / Liquid biopsies come of age : towards implementation of circulating tumour DNA / Nat Rev Cancer 17:223~238
Merker JD / 2018 / Circulating tumor DNA analysis in patients with cancer : American Society of Clinical Oncology and College of American Pathologists Joint Review / J Clin Oncol 36:1631~1641
Advani HV / 2017 / Challenges in non-invasive prenatal screening for sub-chromosomal copy number variations using cell-free DNA / Prenat Diagn 37:1067~1075
Heitzer E / 2015 / Circulating tumor DNA as a liquid biopsy for cancer / Clin Chem 61:112~123
Meddeb R / 2019 / Guidelines for the preanalytical conditions for analyzing circulating cell-free DNA / Clin Chem 65:623~633
Merker JD / 2018 / Circulating tumor DNA analysis in patients with cancer : American Society of Clinical Oncology and College of American Pathologists Joint Review / J Clin Oncol 36:1631~1641
Advani HV / 2017 / Challenges in non-invasive prenatal screening for sub-chromosomal copy number variations using cell-free DNA / Prenat Diagn 37:1067~1075
Heitzer E / 2015 / Circulating tumor DNA as a liquid biopsy for cancer / Clin Chem 61:112~123
Meddeb R / 2019 / Guidelines for the preanalytical conditions for analyzing circulating cell-free DNA / Clin Chem 65:623~633
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