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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 835 - 845 (11page)
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초록· 키워드
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Background: One of the long-standing problems of myoblasts in vitro expansion is slow cell migration and this causes fibroblast population to exceed myoblasts. In this study, we investigated the synergistic effect of laminin and epidermal growth factor (EGF) on co-cultured myoblasts and fibroblasts for cell attachment, proliferation and migration. Methods: Skeletal human muscle cells were cultured in four different conditions; control, EGF, laminin (Lam) and laminin EGF (Lam + EGF). Using live imaging system, their cellular properties; attachment, migration and growth were exposed to Rho kinase inhibitor, Y-27632, and EGF-receptor (EGF-R) inhibitor, gefitinib were measured. Results: Myoblast migration and proliferation was enhanced significantly by synergistic stimulation of laminin and EGF (0.61 ± 0.14 µm/min, 0.008 ± 0.001 h−1) compare to that by EGF alone (0.26 ± 0.13 µm/min, 0.004 ± 0.0009 h−1). However, no changes in proliferation and migration were observed for fibroblasts among the culture conditions. Inhibition of Rho kinase resulted in the increase of the myoblast migration on the laminin-coated surface with EGF condition (0.64 ± 0.18 µm/min). Compared to the untreated conditions, myoblasts cultured on the laminin-coated surface and EGF demonstrated elongated morphology, and average cell length increase significantly. In contrast, inhibition of EGF-R resulted in the decrease of myoblast migration on the laminin coated surface with EGF supplemented condition (0.43 ± 0.05 µm/min) in comparison to the untreated control (0.53 ± 0.05 µm/min). Conclusion: Laminin and EGF preferentially enhance the proliferation and migration of myoblasts, and Rho kinase and EGF-R play a role in this synergistic effect. These results will be beneficial for the propagation of skeletal muscle cells for clinical applications.
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참고문헌 (46)
참고문헌 신청
Mauro A / 1961 / Satellite cell of skeletal muscle fibers / J Biophys Biochem Cytol 9:493~495
Montarras D / 2005 / Direct isolation of satellite cells for skeletal muscle regeneration / Science 309:2064~2067
Sherwood RI / 2004 / Isolation of adult mouse myogenic progenitors : functional heterogeneity of cells within and engrafting skeletal muscle / Cell 119:543~554
Collins CA / 2005 / Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche / Cell 122:289~301
McGarvey ML / 1984 / Synthesis and effects of basement membrane components in cultured rat Schwann cells / Dev Biol 105:18~28
Montarras D / 2005 / Direct isolation of satellite cells for skeletal muscle regeneration / Science 309:2064~2067
Sherwood RI / 2004 / Isolation of adult mouse myogenic progenitors : functional heterogeneity of cells within and engrafting skeletal muscle / Cell 119:543~554
Collins CA / 2005 / Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche / Cell 122:289~301
McGarvey ML / 1984 / Synthesis and effects of basement membrane components in cultured rat Schwann cells / Dev Biol 105:18~28
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