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논문 기본 정보

자료유형
학술저널
저자정보
Hyo-Jung Kim (University of Ulsan) Iel-Yong Sung (University of Ulsan) Yeong-Cheol Cho (University of Ulsan) Min-Su Kang (Changwon Gyeongsang National University Hospital) Gyu-Jin Rho (Gyeongsang National University) June-Ho Byun (Gyeongsang National University) Won-Uk Park (Jinju Health College) Myeong-Gyun Son (Hanil Hospital) Bong-Wook Park (Hanil Hospital) Hyeon-Jeong Lee (Gyeongsang National University) Young-Hoon Kang (Gyeongsang National University)
저널정보
한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제16권 제5호
발행연도
2019.1
수록면
513 - 523 (11page)

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BACKGROUND: Enhancement and maintenance of the stemness of mesenchymal stem cells (MSCs) is one of the most important factors contributing to the successful in vivo therapeutic application of these cells. In this regard, threedimensional (3D) spheroid formation has been developed as reliable method for increasing the pluripotency of MSCs. Moreover, using a new protocol, we have previously shown that dental tissues of extracted wisdom teeth can be effectively cryopreserved for subsequent use as a source of autologous stem cells. The main purpose of this study is to analyze the stemness and in vitro osteogenic differentiation potential of 3D spheroid dental MSCs compared with conventional monolayer cultured MSCs. METHODS: In this study, MSC-characterized stem cells were isolated and cultured from long-term cryopreserved dental follicles (hDFSCs), and then 2D hDFSCs were cultured under 3D spheroid-forming conditions using a newly designed microchip dish. The spheroids (3D hDFSCs) thus produced were investigated and characterized with respect to stemness, MSC marker expression, apoptosis, cell cycle analysis, extracellular matrix (ECM) production, and osteogenic and adipogenic differentiation properties. RESULTS: In terms of MSC and senescence markers, spheroid cells showed no difference when compared with 2D hDFSCs; however, 3D hDFSCs were observed to have a higher proportion of cell cycle arrest and a larger number of apoptotic cells. Moreover, spheroids showed substantially increased levels of pluripotency marker (early transcription factors) and ECM protein expression. Compared with 2D hDFSCs, there was also a notable enhancement in the osteogenic induction potential of spheroids, although no differences were observed with respect to in vitro adipogenesis. CONCLUSION: To the best of our knowledge, this is the first study to demonstrate the application of a spheroid culture system for dental follicle-derived stem cells using a microchip dish. Although further studies are needed, including in vivo transplantation, the results obtained in this study indicate that spheroid hDFSCs derived from cryopreserved dental follicle tissues could be used as a valuable source of autologous stem cells for bone tissue regeneration.
#Mesenchymal stem cells Dental follicle stem cells Spheroid Stemness Osteogenesis

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참고문헌 (40)

참고문헌 신청
Klingemanna H / 2008 / Mesenchymal stem cells sources and clinical applications / Transfus Med Hemother 35:272~277 google schola Ben-Ami E / 2011 / Mesenchymal stem cells as an immunomodulatory therapeutic strategy for autoimmune diseases / Autoimmun Rev 10:410~415 google schola Kwon A / 2016 / Tissue-specific differentiation potency of mesenchymal stromal cells from perinatal tissues / Sci Rep 6:23544 google schola Yagi H / 2010 / Mesenchymal stem cells : mechanisms of immunomodulation and homing / Cell Transplant 19:667~679 google schola Hsiao ST / 2012 / Comparative analysis of paracrine factor expression in human adult mesenchymal stem cells derived from bone marrow, adipose, and dermal tissue / Stem Cells Dev 21:2189~2203 google schola

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