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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 395 - 403 (9page)
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BACKGROUND: Silica particles (SPs) induce cell proliferation and osteogenic differentiation. We reported that SPs in the scaffold induced early stage osteogenic differentiation. METHODS: A polycaprolactone (PCL) scaffold was fabricated with a 10 wt% SPs. The surface of PCL scaffold was coated with a 10 lg/mL collagen solution. Next, the scaffold was conjugated with 2 lM SPs, 2 lg/mL bone morphogenetic protein 2 (BMP2), or 2 lM BMP2-conjugated SPs (BCSPs). Green fluorescent protein-coupled BMP2 was applied to fabricate the scaffold. The fluorescence intensity was analyzed by confocal microscopy. The mRNA levels of the early osteogenic differentiation marker, alkaline phosphatase (ALP), were analyzed by real-time quantitative polymerase chain reaction. Levels of BMP2, RUNX2, ERK1/2, and AKT were assessed by western blotting. RESULTS: ALP mRNA levels were significantly higher in the BCSP-conjugated scaffold than in the other scaffolds. In the early stage of osteogenic differentiation, the protein levels of BMP2, RUNX2, ERK1/2, and AKT in cells were significantly higher in the BCSP-conjugated scaffold than in other scaffolds. Thus, the BCSP composite scaffold induced rapid osteogenic differentiation. CONCLUSION: These results suggest that BCSP composite can be used to promote early stage osteogenic differentiation and show promise as a material for use in scaffolds for bone regeneration.
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참고문헌 (36)
참고문헌 신청
Zhou X / 2015 / BMP-2derived peptide and dexamethasone incorporated mesoporous silica nanoparticles for enhanced osteogenic differentiation of bone mesenchymal stem cells / ACS Appl Mater Interfaces 7:15777~15789
Yang XB / 2006 / Evaluation of human bone marrow stromal cell growth on biodegradable polymer/bioglass composites / Biochem Biophys Res Commun 342:1098~1107
Webster TJ / 2012 / Anti-infective and osteointegration properties of silicon nitride, poly(ether ether ketone), and titanium implants / Acta Biomater 8:4447~4454
Wu C / 2005 / Preparation and characteristics of a calcium magnesium silicate(bredigite)bioactive ceramic / Biomaterials 26:2925~2931
Shie MY / 2012 / Effects of altering the Si/Ca molar ratio of a calcium silicate cement on in vitro cell attachment / Int Endod J 45:337~345
Yang XB / 2006 / Evaluation of human bone marrow stromal cell growth on biodegradable polymer/bioglass composites / Biochem Biophys Res Commun 342:1098~1107
Webster TJ / 2012 / Anti-infective and osteointegration properties of silicon nitride, poly(ether ether ketone), and titanium implants / Acta Biomater 8:4447~4454
Wu C / 2005 / Preparation and characteristics of a calcium magnesium silicate(bredigite)bioactive ceramic / Biomaterials 26:2925~2931
Shie MY / 2012 / Effects of altering the Si/Ca molar ratio of a calcium silicate cement on in vitro cell attachment / Int Endod J 45:337~345
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