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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 175 - 182 (8page)
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The aim of this study is to quantify caffeic acid derivatives in Echinacea purpurea which has been used to as an immunomodulatory agent in various inflammatory and viral diseases. The root of Echinacea purpurea was extracted with methanol or 70%(v/v) methanol at different extraction conditions. The analysis was executed on an Eclipse plus-C18 column (4.6 mm × 150 mm, 5 μm) at 30 ℃ by gradient elution with a mobile phase of 0.085% (v/v) phosphoric acid in water and acetonitrile. The flow rate was 0.75 mL/min and detection was applied at 330 nm. Chromatographic separations of caffeic acid derivatives such as caftaric acid, caffeic acid and cichoric acid were exhibited and the correlation coefficients of caffeic acid derivatives in the test ranges were 0.9990~1.0000. The limits of detection and quantification values of the caftaric acid, caffeic acid and cichoric acid were 0.0068~0.0592 and 0.0227~0.1974 μg/mL, respectively. Quantitative analysis showed that caftaric acid, caffeic acid and cichoric acid were 6.751~7.440 mg/g, 0.428~ 0.451 mg/g and 10.875~12.446 mg/g in Echinacea purpurea by extraction with 70% methanol at room temperature, 40 ℃ and 40 ℃ with ultrasound treatment for 2 h, respectively.
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참고문헌 (21)
참고문헌 신청
이준경 / 2002 / 암세포에 대한 Echinacea angustifolia 순차 용매 추출물의 세포독성 효과 / 한국식품과학회지 34(1):123~127
최인영 / 2020 / 에키나시아 흰가루병을 일으키는 Podosphaera xanthii 동정 / 한국균학회지 48(3):337~343
Bauer, R. / 1991 / Economic and medicinal plant research / Academic Press :253~321
Bruni, R / 2018 / Analytical methods for the study of bioactive compounds form medicinally used Echinacea species / Journal of Pharmaecutical and Biomedical Analysis 160:443~477
Cheminat, A / 1988 / Caffeoyl conjugates from Echinacea species : Structures and biological activity / Phytochemistry 27(9):2787~2794
최인영 / 2020 / 에키나시아 흰가루병을 일으키는 Podosphaera xanthii 동정 / 한국균학회지 48(3):337~343
Bauer, R. / 1991 / Economic and medicinal plant research / Academic Press :253~321
Bruni, R / 2018 / Analytical methods for the study of bioactive compounds form medicinally used Echinacea species / Journal of Pharmaecutical and Biomedical Analysis 160:443~477
Cheminat, A / 1988 / Caffeoyl conjugates from Echinacea species : Structures and biological activity / Phytochemistry 27(9):2787~2794
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