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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 308 - 316 (9page)
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We report a dramatic improvement in the detection sensitivity of electrochemical immunosensors through the use of a magnetic nanoparticle for immunoassay reactions and an ITO working electrode for measurement of the electrochemical enzymatic reaction signals. In general, electrochemical sensors developed for immunoassay require the immobilization of capture antibodies on the surface of the working electrode. Under these circumstances, the working electrode comes into direct contact with various biological samples, and unwanted non-specific binding inevitably interferes with the detection signal. Moreover, chemical surface treatment of the working electrode to immobilize capture antibodies degrades the quality of the electrochemical signals during target determination. Here, we address these issues by immobilizing capture antibodies on the surface of magnetic nanoparticles and separating the surface for measurement of electrochemical signals from the immune reactions. By avoiding direct contact with biological materials, the surface of the working electrode could not be contaminated and could be maintained clean. We also provide experimental evidence that these novel immunoassay platforms benefit from a reduction in the noise signal. Indeed, the signal-to-noise ratio, an essential characteristic of quantitative detection, could be improved by approximately 11.8-fold compared with the electrochemical immunoassay of conventional surface modification approach, while retaining an excellent coefficient of variation (≤ 18%). The method was applied to the practical immunosensing for matrix metalloproteinase- 9 (MMP-9), the bladder cancer marker in the urine, and exhibited excellent performance.
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참고문헌 (30)
참고문헌 신청
Glavan, A. C. / 2014 / Folding analytical devices for electrochemical ELISA in hydrophobic RH paper / Anal. Chem 86:11999~12007
Kaushik, A. / 2015 / Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients / Int. J. Nanomed. 10:677~685
Kim, H. / 2019 / A new point-of-care test for the diagnosis of infectious diseases based on multiplex lateral flow immunoassays / Analyst 144:2460~2466
Pacheco, J. G. / 2018 / Molecularly imprinted electrochemical sensor for the point-of-care detection of a breast cancer biomarker (CA 15-3) / Sens. Actuators, B 256:905~912
Quesada-González, D. / 2018 / Nanomaterial-based devices for point-of-care diagnostic applications / Chem. Soc. Rev 47:4697~4709
Kaushik, A. / 2015 / Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients / Int. J. Nanomed. 10:677~685
Kim, H. / 2019 / A new point-of-care test for the diagnosis of infectious diseases based on multiplex lateral flow immunoassays / Analyst 144:2460~2466
Pacheco, J. G. / 2018 / Molecularly imprinted electrochemical sensor for the point-of-care detection of a breast cancer biomarker (CA 15-3) / Sens. Actuators, B 256:905~912
Quesada-González, D. / 2018 / Nanomaterial-based devices for point-of-care diagnostic applications / Chem. Soc. Rev 47:4697~4709
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