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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 148 - 157 (10page)
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초록· 키워드
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Despite the diverse roles of singlet oxygen ( 1 O2) in plants and animals, simple detection of 1 O2 has been limited due to technical difficulties. Here we report a colorimetric method for visualizing the generation and suppression of 1 O2 using a protein photosensitizer, mini singlet oxygen generator (miniSOG). Upon external light irradiation, miniSOG strongly produced 1 O2 and oxidized a universal chromogenic reagent, tetramethylbenzidine, leading to a rapid color change in solution. We used this finding to demonstrate the 1 O2 scavenging effects of well-known antioxidants and of natural compounds extracted from plants. Owing to its high usability and simplicity, our developed assay platform will be useful for screening potential scavengers for reactive oxygen species and will promote the development of various antioxidants.
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등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Krieger-Liszkay, A. / 2008 / Singlet oxygen production in photosystem II and related protection mechanism / Photosynth. Res. 98:551~564
Ledford, H. K. / 2007 / Acclimation to singlet oxygen stress in Chlamydomonas reinhardtii / Eukaryotic Cell 6:919~930
Onyango, A.N. / 2016 / Endogenous Generation of Singlet Oxygen and Ozone in Human and Animal Tissues:Mechanisms, Biological Significance, and Influence of Dietary Components / Oxid. Med. Cell. Longevity 2016:2398573
Triantaphylides, C. / 2009 / Singlet oxygen in plants: production, detoxification and signaling / Trends Plant Sci 14:219~228
Waszczak, C. / 2018 / Reactive Oxygen Species in Plant Signaling / Annu. Rev. Plant Biol. 69:209~236
Ledford, H. K. / 2007 / Acclimation to singlet oxygen stress in Chlamydomonas reinhardtii / Eukaryotic Cell 6:919~930
Onyango, A.N. / 2016 / Endogenous Generation of Singlet Oxygen and Ozone in Human and Animal Tissues:Mechanisms, Biological Significance, and Influence of Dietary Components / Oxid. Med. Cell. Longevity 2016:2398573
Triantaphylides, C. / 2009 / Singlet oxygen in plants: production, detoxification and signaling / Trends Plant Sci 14:219~228
Waszczak, C. / 2018 / Reactive Oxygen Species in Plant Signaling / Annu. Rev. Plant Biol. 69:209~236
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