지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 174 - 181 (8page)
이용수
초록· 키워드
오류제보하기
Although lab-on-a-chip is a promising platform for point-of-care-testing (POCT) applications, its commercialization has been hampered due to complex fabrication processes or the need for additional lab instruments. In this study, tubing-based colourimetric and volumetric enzyme-linked immunosorbent assay (ELISA) methods were developed for application in POCT of cortisol and Salmonella typhimurium, respectively. Different solutions required for each assay were contained in a piece of tubing, forming different aqueous layers that were separated by immiscible organic layers. The assay was carried out by moving antibody-functionalized, solid-phase magnetic particles through the layers with a hand-operated magnet. This method enabled detection of cortisol at a low detection limit of 0.1 ng/mL within 12 min and detection of S. typhimurium down to 102 cfu/sample within 1 h. The performance of the assay methods was comparable to or better than that of conventional ELISA despite the assay systems and methods being extremely simple, thus demonstrating its potential for POCT application.
목차
등록된 정보가 없습니다.
참고문헌 (27)
참고문헌 신청
Bange, A. / 2005 / Microfluidic immunosensor systems / Biosens. Bioelectron 20:2488~2503
Haeberle, S. / 2007 / Microfluidic platforms for lab-on-a-chip applications / Lab Chip 7:1094~1110
Dincer, C. / 2017 / Multiplexed Point-of-Care Testing –xPOCT / Trends Biotechnol 35:728~742
Yáñez-Sedeño, P. / 2017 / Multiplexed Electrochemical Immunosensors for Clinical Biomarkers / Sensors 17:965
Chin, C. D. / 2012 / Commercialization of microfluidic point-of-care diagnostic devices / Lab Chip 12:2118~2134
Haeberle, S. / 2007 / Microfluidic platforms for lab-on-a-chip applications / Lab Chip 7:1094~1110
Dincer, C. / 2017 / Multiplexed Point-of-Care Testing –xPOCT / Trends Biotechnol 35:728~742
Yáñez-Sedeño, P. / 2017 / Multiplexed Electrochemical Immunosensors for Clinical Biomarkers / Sensors 17:965
Chin, C. D. / 2012 / Commercialization of microfluidic point-of-care diagnostic devices / Lab Chip 12:2118~2134
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of Enzyme-linked Immunosorbent Assays (ELISA) on Lateral Flow Biosensor for miRNA Detection
한국생물공학회 학술대회
2015 .04
-
Comprehensive immunological viral detection on micro system
한국생물공학회 학술대회
2017 .10
-
Enhanced Enzyme-Linked Immunosorbent Assay with BC-MAP as a Biolinker for Antibody Immobilization
한국생물공학회 학술대회
2024 .09
-
Characterization of Binding Affinity of Growth Factor Receptor Targeting Polypeptides by Enzyme-linked Immunosorbent Assay
한국고분자학회 학술대회 연구논문 초록집
2016 .04
-
Development of Inkless Visual Volumetric Chip for Point-of-care Testing
한국생물공학회 학술대회
2021 .10
-
Development of Lateral Flow Assays with Magnetic Nanoparticles for Quantitative Antibody Detection
대한기계학회 춘추학술대회
2022 .05
-
Protection of Mice from Critical Damage by the Infection of Salmonella Typhimurium
한국생물공학회 학술대회
2018 .10
-
Fabrication of a POCT platform integrating microfluidic chips and scatter light-based optical biosensors
한국생산제조학회 학술발표대회 논문집
2024 .07
-
Development and comparison of efficient DNA, RNA extraction platforms for POCT (point-of-care test)
대한기계학회 춘추학술대회
2020 .08
-
POCT 용 마이크로칩 제작
한국생산제조학회 학술발표대회 논문집
2024 .07
-
현장진단 적용을 위한 혈액분리 바이오칩의 개발
한국생산제조학회 학술발표대회 논문집
2022 .07
-
Detection of Salmonella typhimurium by Antibody/ Enzyme-Conjugated Magnetic Nanoparticles
BioChip Journal
2015 .01
-
Angular-based Quantitative Assay in 3D Paper-based Analytical Device
한국생물공학회 학술대회
2021 .04
-
Fabrication of 3D Paper-based Microfluidic Chip that Can Control Fluid Flow Without External Device and its Application for Breast Cancer Diagnosis Using ELISA
한국생물공학회 학술대회
2020 .06
-
Surface Enhanced Vibrational Detection Array for Detecting Cortisol Hormone
한국생물공학회 학술대회
2023 .04
-
Digital colorimetric assay on three-dimensional paper-based microfluidic device
한국생물공학회 학술대회
2018 .10
-
Development of an assay system for metalloproteinase-1
한국생물공학회 학술대회
2019 .04
-
Development of a Protease assay Method Via Engineering of Procaspase-3 Enzymes
한국생물공학회 학술대회
2017 .04
-
Polygalacturonase를 검출하기 위한 종이 기반의 효소결합 면역반응 센서 제작
센서학회지
2021 .09
-
Bayesian approach for prediction of primary water stress corrosion cracking in Alloy 690 steam generator tubing
Nuclear Engineering and Technology
2022 .09
이 논문의 저자 정보
최근 본 자료
댓글(0)
0