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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Background: Beta-carotene (BC) is a carotenoid which exerts anti-cancer effects in several types of cancer, including colorectal cancer. Epigenetic modifications of genes, such as histone deacetylation and DNA hypermethylation, have also been detected in various types of cancer. To understand the molecular mechanism underlying cancer preventive and therapeutic effects of BC, microRNAs (miRNAs), histone acetylation, and global DNA methylation in colon cancer stem cells (CSCs) were investigated. Methods: HCT116 colon cancer cells positive for expression of CD44 and CD133 were sorted by flow cytometry and used in subsequent experiments. Cell proliferation was examined by the MTT assay and self-renewal capacity was analyzed by the sphere formation assay. The miRNA sequencing array was used to detect miRNAs regulated by BC. Histone acetylation levels were measured by the Western blot analysis. mRNA expression of DNA methyltransferases (DNMTs) was examined by qPCR and global DNA methylation levels were determined by enzyme-linked immunosorbent assay. Results: Treatment of CD44+CD133+ colon CSCs with BC caused a reduction in both cell proliferation and sphere formation. Analysis of the miRNA sequencing array showed that BC regulated expression of miRNAs associated with histone acetylation. Histone H3 and H4 acetylation levels were elevated by BC treatment. In addition, BC treatment down-regulated DNMT3A mRNA expression and global DNA methylation in colon CSCs. Conclusions: These results suggest that BC regulates epigenetic modifications for its anti-cancer effects in colon CSCs. (J Cancer Prev 2019;24:224-232)
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참고문헌 (50)
참고문헌 신청
Gloria NF / 2014 / Lycopene and beta-carotene induce cell-cycle arrest and apoptosis in human breast cancer cell lines / Anticancer Res 34:1377~1386
Park Y / 2015 / β-Carotene-induced apoptosis is mediated with loss of Ku proteins in gastric cancer AGS cells / Genes Nutr 10:467
Palozza P / 2002 / Induction of cell cycle arrest and apoptosis in human colon adenocarcinoma cell lines by beta-carotene through down-regulation of cyclin A and Bcl-2 family proteins / Carcinogenesis 23:11~18
Burton GW / 1984 / beta-Carotene: an unusual type of lipid antioxidant / Science 224:569~573
Santos MS / 1996 / Natural killer cell activity in elderly men is enhanced by beta-carotene supplementation / Am J Clin Nutr 64:772~777
Park Y / 2015 / β-Carotene-induced apoptosis is mediated with loss of Ku proteins in gastric cancer AGS cells / Genes Nutr 10:467
Palozza P / 2002 / Induction of cell cycle arrest and apoptosis in human colon adenocarcinoma cell lines by beta-carotene through down-regulation of cyclin A and Bcl-2 family proteins / Carcinogenesis 23:11~18
Burton GW / 1984 / beta-Carotene: an unusual type of lipid antioxidant / Science 224:569~573
Santos MS / 1996 / Natural killer cell activity in elderly men is enhanced by beta-carotene supplementation / Am J Clin Nutr 64:772~777
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