Knockdown of eIF3a attenuated cell growth in K1 human thyroid cancer cells

Xucai Zheng; Shengying Wang; Shikai Hong; Jianjun Liu; Chenghao Jiang

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자료유형: 학술저널

저자정보: Xucai Zheng (Anhui Provincial Cancer Hospital) Shengying Wang (Anhui Provincial Cancer Hospital) Shikai Hong (Anhui Provincial Cancer Hospital) Jianjun Liu (Anhui Provincial Cancer Hospital) Chenghao Jiang (Anhui Provincial Cancer Hospital)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.43 No.4

발행연도: 2021.1

수록면: 379 - 388 (10page)

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Background In ribosome establishment and the initiation of translation, eukaryotic translation initiation factor (eIF) 3a is a pivotal functional subunit of the eIF3 complex. In various cancer types, abnormal eIF3a expression plays an important role in tumorigenesis. Objective We aimed to explore the role of eIF3a in human thyroid cancer (TC). Material and methods The expression of eIF3a was determined in TC tissues by qRT-PCR and immunohistochemistry (IHC) assay, respectively. In addition, the expression of eIF3a in K1 and BCPAP cells were detected by qRT-PCR. Cell proliferation, cell cycle, and cell apoptosis were assessed after eIF3a knockdown in K1 in cell line. Results The expression of eIF3a mRNA was high in TC tissues and cancer cell lines. Moreover, eIF3a expression in TC tissues indicated that high eIF3a level was associated with tumor grade. In addition, eIF3a knockdown resulted in a significantly decrease in cell proliferation and increased the apoptosis of K1 cells. Cell cycle was arrested in both the S and G2/M phase. The levels of phosphorylated ERK1/2 and surviving were decreased after eIF3a knockdown. Conclusion Our study suggested that eIF3a contributed to TC cell proliferation. It may be a promising target for gene therapy in human thyroid cancer.

#Apoptosis · Proliferation · Thyroid cancer · ERK1/2 · Survivin

참고문헌 (42)

참고문헌 신청

Are C / 2006 / Anaplastic thyroid carcinoma : biology, pathogenesis, prognostic factors, and treatment approaches / Ann Surg Oncol 13(4):453~464

Asano K / 1997 / Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly / J Biol Chem 272(43):27042~27052

Aylett CH / 2015 / Structure of a yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex / Nat Struct Mol Biol 22(3):269~271

Bachmann F / 1997 / Cloning of a novel protein overexpressed in human mammary carcinoma / Cancer Res 57(5):988~994

Bai L / 2015 / ERK1/2 promoted proliferation and inhibited apoptosis of human cervical cancer cells and regulated the expression of c-Fos and c-Jun proteins / Med Oncol 32(3):57

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