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논문 기본 정보

자료유형
학술저널
저자정보
Hae Ri Kim (Kangwon National University) Kyu Jin Sa (Kangwon National University) Min Nam‑Gung (Maize Research Institute) Ki Jin Park (Maize Research Institute) Si‑Hwan Ryu (Maize Research Institute) Chang Yeun Mo (Kangwon National University) Ju Kyong Lee (Kangwon National University)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.43 No.1
발행연도
2021.1
수록면
79 - 90 (12page)

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Background Association mapping has been advocated as the method of choice for identifying loci involved in the inheritance of complex traits in crop species. This method involves identifying markers with significant differences in allele frequency between individuals with a phenotype of interest and a set of unrelated control individuals. Objective The purpose of our study is not only to investigate the genetic diversity and relationships of the basic molecular markers of near-isogenic lines (NILs) of waxy maize, but it is also to identify molecular markers related to interesting seed characteristics including 4 seed quantity traits and 4 seed phenotypic traits using association analysis with population structure. Methods We performed association mapping of 200 SSR markers and 8 seed characteristics among 10 NILs of waxy maize and two parental lines (HW3, HW9) (recurrent parent) of “Mibaek 2” variety. Results In population structure and cluster analysis, the 10 NILs and two parental lines were divided into two groups. Seven inbred lines, including HW3, were assigned to Group I. Group II contained 5 inbred lines, including HW9. In addition, we found that 32 SSR markers associate with 8 seed characteristics in 10 NILs. In particular, five SSR markers (umc1986, umc1747, umc2275, phi078, umc1366) were together associated with more than one seed characteristics such as EL, 100 KW, SCC, R, L*, and V. Conclusion This study demonstrated the utility of SSR analysis for studying GD, population structure, and association mapping in 10 NILs and two parental lines (HW3, HW9) of “Mibaek 2” variety.

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