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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 1,407 - 1,417 (11page)
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Background: Low temperature (LT) or cold stress is a major environmental stress that seriously affects plant growth and development, limiting crop productivity. Cold shock domain proteins (CSDPs), which are present in most living organism, are involved in RNA metabolisms influencing abiotic stress tolerance. Objective: The aims of this study are to identify target gene for LT-tolerance, like CSDPs, characterize genetics, and develop molecular marker distinguishing LT-tolerance in cabbage (Brassica oleracea var. capitata). Methods: Semi-quantitative RT-PCR or qRT-PCR was used in gene expression study. LT-tolerance was determined by electrolyte leakage and PCR with allelic specific primers. Results: Allelic variation was found in BoCSDP5 coding sequence (CDs) between LT-tolerant (BN106 and BN553) and -susceptible inbred lines (BN107 and BN554). LT-tolerant inbred lines contained variant type of BoCSDP5 (named as BoCSDP5v) which encodes extra CCHC zinc finger domain at C-terminus. Association of LT-tolerance with BoCSDP5v was confirmed by electrolyte leakage and segregation using genetic population derived from BN553 and BN554 cross. Allelic variation in BoCSDP5 gene does not influence the rate of gene expression, but produces different proteins with different number of CCHC zinc finger domains. LT-tolerance marker designed on the basis of polymorphism between BoCSDP5 and BoCSDP5v was confirmed with samples used in previous B. oleracea CIRCADIAN CLOCK ASSOCIATED 1 (BoCCA1) marker validation. Conclusions: LT-tolerant allele (BoCSDP5v) is dominant and independent of CBF pathway, and sufficient to generate molecular markers to identify LT-tolerant cabbage when it is used in combination with another marker, like BoCCA1-derived one. Production and analysis of overexpressing plants of BoCSDP1, BoCSDP3, BoCSDP5 and BoCSDP5v will be required for elucidating the function of CCHC zinc finger domains in LT-tolerance.
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참고문헌 (60)
참고문헌 신청
Aceituno-Valenzuela U / 2020 / Genome-wide analysis of CCHC-type zinc finger (ZCCHC) proteins in yeast, Arabidopsis, and humans / Cell Mol Life Sci
Ahmed NU / 2015 / Identification and expression analysis of cold and freezing stress responsive genes of Brassica oleracea / Gene 554:215~223
Ambrosone A / 2012 / Beyond transcription : RNA-binding proteins as emerging regulators of plant response to environmental constraints / Plant Sci 182:12~18
Budkina KS / 2020 / Cold shock domain proteins : structure and interaction with nucleic acids / Biochemistry 85:S1~S19
Burd CG / 1994 / Conserved structures and diversity of functions of RNA-binding proteins / Science 269:23074~23078
Ahmed NU / 2015 / Identification and expression analysis of cold and freezing stress responsive genes of Brassica oleracea / Gene 554:215~223
Ambrosone A / 2012 / Beyond transcription : RNA-binding proteins as emerging regulators of plant response to environmental constraints / Plant Sci 182:12~18
Budkina KS / 2020 / Cold shock domain proteins : structure and interaction with nucleic acids / Biochemistry 85:S1~S19
Burd CG / 1994 / Conserved structures and diversity of functions of RNA-binding proteins / Science 269:23074~23078
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