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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 154 - 154 (1page)
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Ethylene glycol (EG) is a bulk chemical that is mainly used as an anti-freezing agent and a raw material in the synthesis of plastics. Production of commercial EG mostly relies on chemical synthesis using fossil resources. Biochemical production of EG from renewable resources may be more sustainable. Herein, we have examined a strategy for the biosynthesis of EG from formaldehyde. First, we selected glyoxylate carboligase from Escherichia coli K-12 (EcGCL) as glycolaldehyde synthase. EcGCL catalyzes the condensation of two molecules formaldehyde into glycolaldehyde with high thermostability. The variants were constructed to enhance the catalytic efficiency by engineering active site and substrate entrance site based on crystal structures and substrate docking simulation. The b ... 전체 초록 보기
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