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Serial crystallography (SX) makes a significant contribution for time-resolved studies and forms the base of structural analysis at room temperature, with minimal radiation damage. Even though X-ray free electron laser provides a femtosecond scale X-ray pulse, high accessibility of synchrotron facilities gives a merit for application of SX experiment. Therefore, we performed serial synchrotron crystallography (SSX) of lysozyme crystals at room-temperature. Both fixed target and injector-based methods were used for SX experiments to determine the structure of lysozyme crystals. Approximately 19,600 and 40,000 diffraction images were collected during 40 and 80 min, for fixed target and injectionbased methods, respectively in the SSX experiments. The 10 Hz synchrotron X-ray radiation of the 11C beamline of the Pohang accelerator laboratory was used and the crystal structures of lysozyme were determined at 1.89 A and 1.80 A resolutions, respectively. These results provide experimental clues for routine SX at room temperature in synchrotrons.
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참고문헌 (48)
참고문헌 신청
Adams, P. D. / 2010 / PHENIX : a comprehensive Python-based system for macromolecular structure solution / Acta Crystallogr D Biol Crystallogr 66:213~221
Anfinsen, C. B. / 1973 / Principles that govern the folding of protein chains / Science 181:223~230
Barty, A. / 2014 / Cheetah: software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data / J Appl Crystallogr 47:1118~1131
Baxter, E. L. / 2016 / High-density grids for efficient data collection from multiple crystals / Acta Crystallogr D Struct Biol 72:2~11
Brockhouse, B. N. / 1995 / Announcement / Solid State Commun 93:471
Anfinsen, C. B. / 1973 / Principles that govern the folding of protein chains / Science 181:223~230
Barty, A. / 2014 / Cheetah: software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data / J Appl Crystallogr 47:1118~1131
Baxter, E. L. / 2016 / High-density grids for efficient data collection from multiple crystals / Acta Crystallogr D Struct Biol 72:2~11
Brockhouse, B. N. / 1995 / Announcement / Solid State Commun 93:471
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