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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 173 - 179 (7page)
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초록· 키워드
오류제보하기
The objective of this study was to evaluate the usefulness of detection of PRRSV and PRRSV-specific antibodies in oral fluids for monitoring of PRRSV infection in endemic farms. The level of PRRSV and anti-PRRSV antibodies in serum and oral fluids was evaluated in five age groups of pigs (6, 9, 12, 16 weeks of age and gilts). The samples (25 serums and 5 oral fluids/per a farm) were collected from 5 different farms endemically infected by PRRSV. Both serum and oral fluid samples were tested for PRRSV by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and for anti-PRRSV antibodies by two commercial PRRSV ELISA kits. ELISA mean s/p ratios (2.98 vs 1.63) and positive rate (84.0% vs 68.8%) of the oral fluid samples showed significantly higher levels but had similar patterns to the seroprofile of the blood samples. The PRRSV positive rate of oral fluid and serum samples was 40.0% and 44.0% respectively. In conclusion, the use of oral fluids for PRRS monitoring in endemic farms is strongly recommended.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Baron T / 1992 / Report on the first outbreaks of the porcine reproductive and respiratory syndrome (PRRS) in France. Diagnosis and viral isolation / Ann Rech Vet 23:161~166
Biernacka K / 2016 / Detection of porcine reproductive and respiratory syndrome virus(PRRSV)and influenza A virus(IAV)in oral fluid of pigs / Res Vet Sci 109:74~80
Cuong NV / 2014 / Serological and virological surveillance for porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and influenza A viruses among smallholder swine farms of the Mekong Delta, Vietnam / J Swine Health Prod 22:224~231
Decorte I / 2015 / Diagnosis of the Lelystad strain of porcine reproductive and respiratory syndrome virus infection in individually housed pigs : comparison between serum and oral fluid samples for viral nucleic acid and antibody detection / J Vet Diagn Invest 27:47~54
Gerber PF / 2013 / Comparison of commercial real-time reverse transcription-PCR assays for reliable, early, and rapid detection of heterologous strains of porcine reproductive and respiratory syndrome virus in experimentally infected or noninfected boars by use of different sample types / J Clin Microbiol 51:547~556
Biernacka K / 2016 / Detection of porcine reproductive and respiratory syndrome virus(PRRSV)and influenza A virus(IAV)in oral fluid of pigs / Res Vet Sci 109:74~80
Cuong NV / 2014 / Serological and virological surveillance for porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and influenza A viruses among smallholder swine farms of the Mekong Delta, Vietnam / J Swine Health Prod 22:224~231
Decorte I / 2015 / Diagnosis of the Lelystad strain of porcine reproductive and respiratory syndrome virus infection in individually housed pigs : comparison between serum and oral fluid samples for viral nucleic acid and antibody detection / J Vet Diagn Invest 27:47~54
Gerber PF / 2013 / Comparison of commercial real-time reverse transcription-PCR assays for reliable, early, and rapid detection of heterologous strains of porcine reproductive and respiratory syndrome virus in experimentally infected or noninfected boars by use of different sample types / J Clin Microbiol 51:547~556
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