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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 262 - 271 (10page)
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초록· 키워드
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Communication among epididymal epithelial cells creates the best luminal condition where spermatozoa mature, transport and are stored. Vacuolar ATPase (V-ATPase) and cytokeratin 5 (KRT5) have been used as signal indicators for clear and basal cells of the epididymal epithelium, respectively, in mice, rats, bats, and pigs; however, these two markers have not yet been described in the epididymis of bulls. Here, we examined the presence and distribution of the B1 subunit of V-ATPase (B1-VATPase) and KRT5 in the distinct regions of adult bovine epididymides, specifically, the caput, corpus, and cauda. Immunofluorescence staining and confocal microscopy showed that narrow shaped-clear cells were placed in the caput and corpus regions of the bovine epididymis; however, they were absent in the cauda epididymis. In addition, B1-VATPase was highly expressed in the cauda spermatozoa; however, it was rarely detected in the caput spermatozoa. On the other hand, KRT5-positive cells, basal cells, were maintained beneath the basal lamina and they had the traditional form with a domeshaped morphology from the caput to cauda region of the bovine epididymis. The co-expression of B1-VATPase and KRT5 was confined to basal cells placed in the basal region of the epithelium. In summary, 1) clear cells were present with region-specific localization, 2) B1-VATPase was present in the corpus and cauda spermatozoa but absent in the caput, 3) co-expressed cells with B1-VATPase and KRT5 were present in the adult bovine epididymis, and 4) B1-VATPase was not a specific marker for clear cells in the bovine epididymis. Therefore, the perfect epididymal luminal condition created by the specific expression and localization patterns of B1-VATPase might be necessary to obtain fertilizing capacity of spermatozoa in the bovine epididymis.
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등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Robaire B / 2006 / Knobil Neill’s physiology of reproduction / Academic Press :1071~1148
Jones RC / 1998 / Evolution of the vertebrate epididymis / J Reprod Fertil Suppl 53:163~181
Carmen Dı́ez-Sánchez / 2003 / Mitochondria from ejaculated human spermatozoa do not synthesize proteins / FEBS Letters 553(1-2):205~208
Julieta Caballero / 2012 / Bovine sperm raft membrane associated Glioma Pathogenesis-Related 1-like protein 1 (GliPr1L1) is modified during the epididymal transit and is potentially involved in sperm binding to the zona pellucida / Journal of Cellular Physiology 227(12):3876~3886
Stefan Koch / 2015 / Post-transcriptional Wnt Signaling Governs Epididymal Sperm Maturation / Cell 163(5):1225~1236
Jones RC / 1998 / Evolution of the vertebrate epididymis / J Reprod Fertil Suppl 53:163~181
Carmen Dı́ez-Sánchez / 2003 / Mitochondria from ejaculated human spermatozoa do not synthesize proteins / FEBS Letters 553(1-2):205~208
Julieta Caballero / 2012 / Bovine sperm raft membrane associated Glioma Pathogenesis-Related 1-like protein 1 (GliPr1L1) is modified during the epididymal transit and is potentially involved in sperm binding to the zona pellucida / Journal of Cellular Physiology 227(12):3876~3886
Stefan Koch / 2015 / Post-transcriptional Wnt Signaling Governs Epididymal Sperm Maturation / Cell 163(5):1225~1236
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