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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 884 - 892 (9page)
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초록· 키워드
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Corticosterone is known as a biological stress index in many species including birds. Feather corticosterone concentration (FCC) has increasingly been used as a measure for chronic stress status in broiler chickens. As sample preparation is the first step of analytical process, different techniques of feather matrix disruption need to be validated for obtaining better result in analysing corticosterone extraction. The current study was a validation of pulverizing the feather by bead beater (BB) and surgical scissors (SS) processing prior to corticosterone extraction in feather of broiler chickens. The type of feather processing prior to the hormone extraction may alter the final output. Thereby, finding a standard method according to laboratory facilities is pivotal. This study carried out to determine the effects of feather pulverization methods on the extraction amount of corticosterone in broiler chickens. Feathers were sampled from four weeks old Ross 308 broiler chickens (n = 12 birds). All broiler chickens were kept under the same environmental condition and had access to feed and water. Feather samples were assigned to one of the following processing methods 1) using a BB for pulverizing and 2) using a SS for chopping into tiny pieces. Each sample was duplicated into two wells during enzyme immunoassay (EIA) analysis to improve the accuracy of the obtained data. The results showed lower standard errors and constant output of FCC by using the BB method compared with the SS method. Overall comparison of FCC showed a significantly higher (p < 0.001) amount of the FCC in the BB compared with the SS. Overall, using the BB method is recommended over the SS method for feather processing due to the ability to homogenize a large number of samples simultaneously, ease of use and greater extraction of feather corticosterone.
목차
등록된 정보가 없습니다.
참고문헌 (31)
참고문헌 신청
Greenhouse Gas Inventory and Research Center / Fourth National Communication of the Republic of Korea under the United Nation Framework Convention on Climate Change
Korea Meteorological Administration / 2018 annual climate report
Allen MR / 2018 / Global Warming of 1.5℃: an IPCC Special Report on the impacts of global warming of 1.5℃ above pre-industrial levels and related global greenhouse gas emission pathways, in the context of strengthening the global response to the threat of climate change, sustainable development, and efforts to eradicate poverty / Intergovernmental Panel on Climate Change :47~92
G. R. Bortolotti / 2008 / Corticosterone in feathers is a long-term, integrated measure of avian stress physiology / Functional Ecology 22(3):494~500
Blas J / 2015 / Sturkie’s avian physiology / Academic Press :769~810
Korea Meteorological Administration / 2018 annual climate report
Allen MR / 2018 / Global Warming of 1.5℃: an IPCC Special Report on the impacts of global warming of 1.5℃ above pre-industrial levels and related global greenhouse gas emission pathways, in the context of strengthening the global response to the threat of climate change, sustainable development, and efforts to eradicate poverty / Intergovernmental Panel on Climate Change :47~92
G. R. Bortolotti / 2008 / Corticosterone in feathers is a long-term, integrated measure of avian stress physiology / Functional Ecology 22(3):494~500
Blas J / 2015 / Sturkie’s avian physiology / Academic Press :769~810
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