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This study presents a simple approach for the assembly of a free-standing conductive electronic nanofilm of single-walled carbon nanotubes (SWNTs) suitable for enzymatic electrochemical biosensors. A large-scale SWNT electronic film was successfully produced by the dialysis of p-Terphenyl-4,4´´-dithiol (TPDT)-treated SWNTs. Furthermore, Horseradish peroxidase (HRP) was immobilized on the TPDT-SWNT electronic film, and the enzymatic detection of hydrogen peroxide (H2O2) was demonstrated without mediators. The detection of H2O2 in the negative potential range (?0.4 V vs. Ag/AgCl) was achieved by direct electron transfer of heme-based enzymes that were immobilized on the TPDT-SWNT electronic film. The SWNT-based biosensor exhibited a wide detection range of H2O2 from 10 μM to 10 mM. The HRP-doped SWNT electronic film achieved a high sensitivity of 342 μmA/mM·cm2 and excellent selectivity against a variety of redox-active interfering substances, such as ascorbic acid, uric acid, and acetaminophen.
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참고문헌 (14)
참고문헌 신청
J. Wang / 2001 / Glucose biosensors : 40 years of advances and challenges / Electroanalysis 13(12):983~988
K. Tonyushkina / 2009 / Glucose meters : a review of technical challenges to obtaining accurate results / J. Diabetes. Sci. Technol. 3(4):971~980
S. W. Lee / 2016 / Direct electron transfer of enzymes in a biologically assembled conductive nanomesh enzyme platform / Adv. Mater. 28(8):1577~1584
X. Yu / 2019 / Turn-on fluorescent detection of hydrogen peroxide and triacetone triperoxide via enhancing interfacial interactions of a blended system / Anal. Chem. 91(11):6967~6970
M. P. Wymann / 1987 / Chemiluminescence detection of H2O2 produced by human-neutrophils during the respiratory burst / Anal. Biochem. 165(2):371~378
K. Tonyushkina / 2009 / Glucose meters : a review of technical challenges to obtaining accurate results / J. Diabetes. Sci. Technol. 3(4):971~980
S. W. Lee / 2016 / Direct electron transfer of enzymes in a biologically assembled conductive nanomesh enzyme platform / Adv. Mater. 28(8):1577~1584
X. Yu / 2019 / Turn-on fluorescent detection of hydrogen peroxide and triacetone triperoxide via enhancing interfacial interactions of a blended system / Anal. Chem. 91(11):6967~6970
M. P. Wymann / 1987 / Chemiluminescence detection of H2O2 produced by human-neutrophils during the respiratory burst / Anal. Biochem. 165(2):371~378
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