Sepsis induces variation of intestinal barrier function in different phase through nuclear factor kappa B signaling

Ying-Ya Cao; Zhong-Han Wang; Qian Xu-Cheng; Qun Chen; Zhen Wang; Wei-Hua Lu

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자료유형: 학술저널

저자정보: Ying-Ya Cao (Department of Intensive Care Unit The First Affiliated Hospital of Wannan Medical College Wuhu 2410) Zhong-Han Wang (Department of Intensive Care Unit The First Affiliated Hospital of Wannan Medical College Wuhu 2410) Qian Xu-Cheng (Department of Intensive Care Unit The First Affiliated Hospital of Wannan Medical College Wuhu 2410) Qun Chen (Department of Intensive Care Unit The First Affiliated Hospital of Wannan Medical College Wuhu 2410) Zhen Wang (Department of Intensive Care Unit The First Affiliated Hospital of Wannan Medical College Wuhu 2410) Wei-Hua Lu (Department of Intensive Care Unit The First Affiliated Hospital of Wannan Medical College Wuhu 2410)

저널정보: 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제25권 제4호

발행연도: 2021.1

수록면: 375 - 383 (9page)

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The intestinal barrier function disrupted in sepsis, while little is known about the variation in different phases of sepsis. In this study, mouse models of sepsis were established by caecal ligation and puncture (CLP). The H&E staining of sections and serum diamine oxidase concentration were evaluated at different timepoint after CLP. TUNEL assay and EdU staining were performed to evaluate the apoptosis and proliferation of intestinal epithelium. Relative protein expression was assessed by Western blotting and serum concentrations of pro-inflammatory cytokines was measured by ELISA. The disruption of intestinal barrier worsened in the first 24 h after the onset of sepsis and gradually recovered over the next 24 h. The percentage of apoptotic cell increased in the first 24 h and dropped at 48 h, accompanied with the proliferative rate of intestinal epithelium inhibited in the first 6 h and regained in the later period. Furthermore, the activity of nuclear factor kappa B (NF-?B) presented similar trend with the intestinal barrier function, shared positive correction with apoptosis of intestinal epithelium. These findings reveal the conversion process of intestinal barrier function in sepsis and this process is closely correlated with the activity of NF- ?B signaling.

#Apoptosis #Cell proliferation #Itestinal epithelium #NF-kappa B #Sepsis

참고문헌 (47)

참고문헌 신청

Rhodes A / 2017 / Surviving Sepsis Campaign : International Guidelines for Management of Sepsis and Septic Shock : 2016 / Intensive Care Med 43:304~377

Gotts JE / 2016 / Sepsis: pathophysiology and clinical management / BMJ 353:i1585

Mayr FB / 2014 / Epidemiology of severe sepsis / Virulence 5:4~11

Mittal R / 2014 / Redefining the gut as the motor of critical illness / Trends Mol Med 20:214~223

Li Q / 2009 / Disruption of tight junctions during polymicrobial sepsis in vivo / J Pathol 218:210~221

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