메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류
인문학
인문학 일반 역사학 철학 종교학 어문학 기타 인문학
사회과학
사회과학 일반 경영학 관광학 교육학 군사학 문헌정보학 법학 사회학 신문방송학 심리과학 정치외교학 지리학 행정학 경제학 무역학 인류학 기타 사회과학
자연과학
자연과학 일반 물리학 생물학 생활과학 수학·통계학 천문학 지구과학 화학 기타 자연과학
공학
공학 일반 건축공학 기계공학 산업공학 재료·에너지공학 전기전자공학 조선해양공학 화학공학 컴퓨터학 기타공학
의약학
의학 일반 간호학 수의학 약학 한의학 기초의학 임상의학 기타 의약학
농수해양학
농수해양학 일반 농학 수산학 식품과학 임학 축산학 해상운송학 기타 농수해양학
예술체육학
예술 일반 디자인 미술 미용 사진 음악학 의상 체육 공연매체예술 기타 예술체육학
복합학
학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

ENG

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
Leal Pinto Sandra Milena (Universidad de Santander Facultad de Ciencias de la Salud Bucaramanga Colombia.) Muehlmann Luis Alexandre (Laboratory of Nanoscience and Immunology Faculty of Ceilandia University of Brasilia Brasilia/DF Br) Ojeda Lucia Liliana Mantilla (Universidad de Santander Facultad de Ciencias de la Salud Bucaramanga Colombia.) Vera Arias Angelica Maria (Centro de Investigacion en Enfermedades Tropicales (CINTROP-UIS) Departamento de Ciencias Basicas E) Cordero Martha Viviana Roa (Universidad de Santander Facultad de Ciencias de la Salud Bucaramanga Colombia.) Santos Maria de Fatima Menezes Almeida (Department of Genetics and Morphology Institute of Biological Science University of Brasilia Brasil) Azevedo Ricardo Bentes (Department of Genetics and Morphology Institute of Biological Science University of Brasilia Brasil) Rivero Patricia Escobar (Centro de Investigacion en Enfermedades Tropicales (CINTROP-UIS) Departamento de Ciencias Basicas E)
저널정보
대한감염학회 Infection and Chemotherapy Infection and Chemotherapy 제53권 제2호
발행연도
2021.1
수록면
342 - 354 (13page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
Background Photodynamic therapy (PDT) using chloroaluminium phthalocyanine (ClAlPc) and paromomycin sulfate (PM) can be effective against New World Leishmania species involved in cutaneous leishmaniasis (CL). The aim of this study is to assay the skin permeation and the antileishmanial effects of a nanoemulsion (NE) containing both ClAlPc and PM in experimental CL by Leishmania (Viannia) braziliensis. Material and Methods Cremophor ELP/castor oil-based NEs were prepared by a low-energy method and characterized for their physicochemical parameters. The NEs were used to deliver both ClAlPc and PM to leishmania cells. The in vitro toxicity of NEs were tested in vitro against L. (V.) braziliensis and THP-1 cells. The in vivo toxicity was assessed in non-infected BALB/c mice. Ex-vivo permeation and retention studies using healthy mice skin were also conducted. Finally, the in vivo activity of NE-PM+ClAlPc after PDT was tested in BALB/c mice infected with parasites. Results NEs are colloidally stable with average droplet diameter of 30 nm, polydispersity index (PDI) below 0.2, and zeta potential near zero. Both promastigotes and intracellular amastigotes treated with NE-PM, NE-ClAlPc and NE-PM+ClAlPc were inhibited at >50%, >95%, >88%, respectively, after PDT with a phototoxic index (PI) >1.2. No skin ClAlPc permeation was observed. In contrast, PM skin permeation was 80-fold higher using PM-loaded NE formulation in comparison to aqueous PM solution. Topical treatment with NE formulations showed no signs of local toxicity or genotoxicity. In addition, concentrations of PM between 27.3 - 292.5 μM/25 mg of tissue were detected in different organs. In vivo, the NE-PM+ClAlPc treatment did not reduce skin lesions. Conclusion The Cremophor ELP/castor oil NE formulation increases the permeation of PM through the skin and can be used to co-deliver PM plus ClAlPc for combined PDT protocols. However, the lack of efficacy in the in vivo model evidences that the therapeutical scheme has to be improved.
#Leishmania (Viannia) braziliensis #Photodynamic therapy #Toxicity #Antileishmanial activity #Permeation

목차

등록된 정보가 없습니다.

참고문헌 (38)

참고문헌 신청
World Health Organization (WHO) / Leishmaniasis google schola Pan American Health Organization (PAHO) / Leishmaniasis. Epidemiological report of the Americas google schola Alvar J / 2012 / Leishmaniasis worldwide and global estimates of its incidence / PLoS One 7:e35671 google schola Chakravarty J / 2019 / Current and emerging medications for the treatment of leishmaniasis / Expert Opin Pharmacother 20:1251~1265 google schola anas A / 2011 / Short-term acetylsalicylic acid (aspirin)use for pain, fever, or colds - gastrointestinal adverse effects: a meta-analysis of randomized clinical trials / Drugs R D 11:277~288 google schola

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0