지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 55 - 67 (13page)
이용수
초록· 키워드
오류제보하기
The colorectal carcinoma-associated protein GA733-2 is one of the representative candidate protein for the development of plant-derived colorectal cancer vaccine. Despite of its signifcant importance for colorectal vaccine development, low efciency of GA733-2 production limits its wide applications. To improve productivity of GA733-2 in plants, we here tested multiple factors that afect expression of recombinant GA733-2 (rGA733-2) and rGA733 fused to fragment crystallizable (Fc) domain (rGA733-Fc) protein. The rGA733-2 and rGA733-Fc proteins were highly expressed when the pBINPLUS vector system was used for transient expression in tobacco plants. In addition, the length of interval between rGA733-2 and left border of T-DNA afected the expression of rGA733 protein. Transient expression analysis using various combinations of Agrobacterium tumefaciens strains (C58C1, LBA4404, and GV3101) and tobacco species (Nicotiana tabacum cv. Xanthi nc and Nicotiana benthamiana) revealed that higher accumulation of rGA733-2 and rGA733-Fc proteins were obtained by combination of A. tumefaciens LBA4404 and Nicotiana benthamiana. Transgenic plants generated by introduction of the rGA733-2 and rGA733-Fc expression cassettes also signifcantly accumulated corresponding recombinant proteins. Bioactivity and stability of the plant-derived rGA733 and rGA733-Fc were evaluated by further in?vitro assay, western blot and N-glycosylation analysis. Collectively, we here suggest the optimal condition for efcient production of functional rGA733-2 protein in tobacco system.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Functional characterization of gibberellin signaling-related genes in Panax ginseng
Journal of Plant Biotechnology
2021 .09
-
Production of Recombinant Human Interleukin-11 (IL-11) in Transgenic Tobacco (Nicotiana tabacum) Plants
Journal of Plant Biotechnology
2016 .12
-
Expression of BMP2-Hydrophobin fusion protein in the tobacco plant and molecular dynamic evaluation of its simulated model
Plant Biotechnology Reports
2021 .01
-
Effect of Nitrogen Deficiency on Recombinant Protein Production and Dimerization and Growth in Transgenic Plants
한국원예학회 학술발표요지
2017 .10
-
Effects of High Protein Diet on Chemical-Induced and on Spontaneous Mouse Colorectal Cancer Development
한국식품영양과학회 학술대회발표집
2019 .10
-
산란계에 편백나무 정유 살포가 닭진드기에 미치는 영향
한국가금학회지
2018 .09
-
Strategies and Innovations to Enhance the Viability of Plant Proteins
한국식품영양과학회 학술대회발표집
2022 .10
-
A robust genome-editing method for wild plant species Nicotiana attenuata
Plant Biotechnology Reports
2020 .01
-
A modified transient gene expression protocol for subcellular protein localization analysis in rice
Plant Biotechnology Reports
2020 .01
-
뼈 연화 고등어(Scomber japonicus) 가공품의 식품성분 특성
한국수산과학회지
2018 .12
-
Optimization of Agrobacterium-mediated transient expression of heterologous genes in spinach
Plant Biotechnology Reports
2017 .12
-
Pathogenesis-related proteins induced by agroinoculationassociated cell wall weakening can be obviated by spray-on inoculation or mannitol ex vivo culture
Plant Biotechnology Reports
2017 .06
-
Production of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activity
Plant Biotechnology Reports
2020 .01
-
Model Plant (Nicotiana benthamiana) Transient Assay and Functional Assay of PgTRx1 Gene Derived from Fermented Wild Ginseng Cultured Root
한국약용작물학회 학술대회논문집
2018 .05
-
쌀단백질의 가치와 이용방법
식품과학과 산업
2019 .03
-
Optimized phos-tag mobility shift assay for the detection of protein phosphorylation in planta
Journal of Plant Biotechnology
2018 .01
-
Development of a “deconstructed” vector based on the genome of grapevine virus A
Plant Biotechnology Reports
2019 .01
-
Plant-made pharmaceuticals: exploring studies for the production of recombinant protein in plants and assessing challenges ahead
Plant Biotechnology Reports
2023 .02
-
Overexpressing fusion proteins of 4-coumaroyl-CoA ligase (4CL) and stilbene synthase (STS) in tobacco plants leading to resveratrol accumulation and improved stress tolerance
Plant Biotechnology Reports
2018 .10
-
Characterization of Type 2A Serine/Threonine Protein Phosphatases Using Transgenic Tobacco
한국원예학회 학술발표요지
2016 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0