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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 725 - 736 (12page)
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초록· 키워드
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Strawberry (Fragaria×ananassa Duch.) is one of the most widely cultivated fruit crop. Anthracnose caused?by Colletotrichum?spp. is a devastating disease of strawberry, causing large-scale strawberry losses worldwide. Chitinases act as defence proteins and are crucial for plant response to pathogens. Here, we isolated a class V Chitinase gene (designed as FnCHIT2, GenBank accession number MN709779) from Chinese wild diploid strawberry Fragaria nilgerrensis Schlecht (F. nilgerrensis), a species that exhibits high tolerance to anthracnose. Gene expression analysis showed that FnCHIT2 expression was highly induced after Colletotrichum gloeosporiodes inoculation and salicylic acid (SA) treatment. Subcellular localization analysis revealed the presence of FnCHIT2 in the plasma membrane. Recombinant FnCHIT2 protein was successfully expressed in E. coli Rosetta (DE3). Furthermore, we transformed FnCHIT2 into Col-0 wild type A. thaliana to perform functional analysis and evaluated the functions of Colletotrichum higginsianum and Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). FnCHIT2 overexpression in A. thaliana showed enhanced resistance to C. higginsianum and Pst DC3000. Enhanced disease resistance of FnCHIT2 transgenic plants to C. higginsianum was correlated with pathogenesis-related gene 1 (AtPR1) and plant defensin 1.2 (AtPDF1.2) gene expression levels. These results provide evidence that?FnCHIT2?may play an important role in response to fungal pathogens in strawberry. Our study provides an important theoretical reference for future strawberry resistance breeding.
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참고문헌 (64)
참고문헌 신청
Abeles FB / 1971 / Preparation and purification of glucanase and chitinase from bean leaves / Plant Physiol 47:129~134
Akhan A / 2004 / Molecular cloning, characterization, and expression analysis of two class II chitinase genes from the strawberry plant / Plant Sci 166:753~762
Amil-Ruiz F / 2016 / Partial activation of SA- and JA-defensive pathways in strawberry upon Colletotrichum acutatum interaction / Front Plant Sci 7:1036
Brotmana Y / 2012 / The LysM Receptor-Like kinase LysM RLK1 is required to activate defense and abiotic-stress responses induced by overexpression of fungal chitinases in Arabidopsis Plants / Nat Plants 5:1113~1124
Cao RH / 2009 / Mycoparasitism of endophytic fungi isolated from reed on soilborne phytopathogenic fungi and production of cell wall-degrading enzymes in vitro / Curr Microbiol 59:584~592
Akhan A / 2004 / Molecular cloning, characterization, and expression analysis of two class II chitinase genes from the strawberry plant / Plant Sci 166:753~762
Amil-Ruiz F / 2016 / Partial activation of SA- and JA-defensive pathways in strawberry upon Colletotrichum acutatum interaction / Front Plant Sci 7:1036
Brotmana Y / 2012 / The LysM Receptor-Like kinase LysM RLK1 is required to activate defense and abiotic-stress responses induced by overexpression of fungal chitinases in Arabidopsis Plants / Nat Plants 5:1113~1124
Cao RH / 2009 / Mycoparasitism of endophytic fungi isolated from reed on soilborne phytopathogenic fungi and production of cell wall-degrading enzymes in vitro / Curr Microbiol 59:584~592
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