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논문 기본 정보

자료유형
학술저널
저자정보
Quyen Van Nguyen (Hanoi National University of?Education) Hang Thi Khuat (Hanoi National University of?Education) Yen?Ngoc Thi Nguyen (Hanoi National University of?Education) Dung Thi Vu (Hanoi National University of?Education) Thu?Ha Bui (Hanoi National University of?Education) 부경환 (Jeju National University)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제14권 제5호
발행연도
2020.1
수록면
575 - 584 (10page)

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Drynaria bonii H. Christ is a medicinal plant that has traditionally been used for the treatment of chronic diseases and tuberculosis. However, D. bonii has not yet been cultivated or propagated. In this study, we optimized the conditions for the spore culture of D. bonii to produce sporophytes. First, we evaluated the efects of various factors, such as temperature, light intensity, potting material, and pH, on spore germination and early gametophyte development. Under optimal culture conditions, 95?99% of the spores germinated within 2?weeks of culture. Next, based on these results, we established a culture system for gametophyte development and sporophyte production. Mature gametophytes frst appeared 5?months post-germination (mpg), and at 12 mpg, the rate of production of heart-shaped gametophytes reached 56.6%. Sporophytes at the early frst-leaf stage frst appeared at 6 mpg, and at 12 mpg, the rate of formation of sporophytes reached 15.4%. Interestingly, 6.3% of all gametophytes produced multiple sporophytes. Additionally, 42?62% of the gametophytes also produced multiple secondary gametophytes, indicating a high potential of D. bonii gametophytes to generate new gametophytes and, subsequently, sporophytes. When transferred to new trays or pots, sporophytes grew well and showed 100% survival. Overall, we conclude that this spore culture system can be successfully used for the propagation of D. bonii sporophytes.

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