Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus                      Inonotus obliquus

He Huihui; Li Yingying; Fang Mingyue; Li Tiantian; Liang Yunxiang; Mei Yuxia

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자료유형: 학술저널

저자정보: He Huihui (State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong A) Li Yingying (State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong A) Fang Mingyue (State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong A) Li Tiantian (State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong A) Liang Yunxiang (State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong A) Mei Yuxia (State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong A)

저널정보: 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제6호

발행연도: 2021.1

수록면: 855 - 866 (12page)

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The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated. After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups. Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source. Molecular weights of four NIOPs (termed as NIOPG, NIOPF, NIOPL, and NIOPGL) were respectively 780.90, 1105.00, 25.32, and 10.28 kDa. Monosaccharide composition analyses revealed that NIOPs were composed of Glc, Man, and Gal at different molar ratios. The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions. In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and antitumor activities of NIOPs were substantially affected by carbon source. qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source. Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.

#Inonotus obliquus #carbon source #liquid fermentation #polysaccharide synthesis #structural properties #activities

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