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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제7호
- 발행연도
- 2020.1
- 수록면
- 1,082 - 1,091 (10page)
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초록· 키워드
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Microbial transglutaminases (MTGs) are widely used in the food industry. In this study, the MTG gene of Streptomyces sp. TYQ1024 was cloned and expressed in a food-grade bacterial strain, Bacillus subtilis SCK6. Extracellular activity of the MTG after codon and signal peptide (SP Ync M) optimization was 20 times that of the pre-optimized enzyme. After purification, the molecular weight of the MTG was 38 kDa and the specific activity was 63.75 U/mg. The optimal temperature and pH for the recombinant MTG activity were 50°C and 8.0, respectively. MTG activity increased 1.42- fold in the presence of β-ME and 1.6-fold in the presence of DTT. Moreover, 18% sodium chloride still resulted in 83% enzyme activity, which showed good salt tolerance. Cross-linking gelatin with the MTG increased the strength of gelatin 1.67 times and increased the thermal denaturation temperature from 61.8 to 75.8°C. The MTG also significantly increased the strength and thermal stability of gelatin. These characteristics demonstrated the huge commercial potential of MTG, such as for applications in salted protein foods.
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참고문헌 (43)
참고문헌 신청
Lorand L / 2003 / Transglutaminases : crosslinking enzymes with pleiotropic functions / Nat. Rev. Mol. Cell Biol 4:140~156
Yokoyama K / 2004 / Properties and applications of microbial transglutaminase / Appl. Microbiol. Biotechnol 64:447~454
Liu S / 2015 / Overproduction of pro-transglutaminase from Streptomyces hygroscopicus in Yarrowia lipolytica and its biochemical characterization / BMC Biotechnol 15:75
Duran R / 1998 / Purification, characterisation, and gene cloning of transglutaminase from Streptoverticillium cinnamoneum CBS 683. 68 / Biochimie 80:313~319
Ando H / 1989 / Purification and characteristics of a novel transglutaminase derived from microorganisms / Agric. Biol. Chem 53:2613~2617
Yokoyama K / 2004 / Properties and applications of microbial transglutaminase / Appl. Microbiol. Biotechnol 64:447~454
Liu S / 2015 / Overproduction of pro-transglutaminase from Streptomyces hygroscopicus in Yarrowia lipolytica and its biochemical characterization / BMC Biotechnol 15:75
Duran R / 1998 / Purification, characterisation, and gene cloning of transglutaminase from Streptoverticillium cinnamoneum CBS 683. 68 / Biochimie 80:313~319
Ando H / 1989 / Purification and characteristics of a novel transglutaminase derived from microorganisms / Agric. Biol. Chem 53:2613~2617
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