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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.7
- 수록면
- 208 - 208 (1page)
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Stress deteriorates the gastrointestinal tract by causing changes in intestinal motility, visceral perception, and the function of the gut barrier. Also, neuronal nitric oxide synthase (nNOS) has various roles as a neurotransmitter and is highly produced in irritable bowel syndrome (IBS) patients" plasma and rectal tissues. We reasoned that in vivo HiBiT system would be applicable to identify the correlation between nNOS and stress response. For this, we generated a genetically modified mouse with a 33 base-pair of HiBiT sequence knock-in into the nNOS gene C-terminal end using CRISPR/Cas9 mediated single-stranded template repair. nNOS concentration was measured in the stomach, small intestine, large intestine, adrenal gland, and hypothalamus tissues after e ... 전체 초록 보기
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