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논문 기본 정보

자료유형
학술저널
저자정보
Dong Ryul Lee (Chonnam National University) Chaw Ei Htwe Maung (Chonnam National University) Tae Gyu Choi (Chonnam National University) Kil Yong Kim (Chonnam National University)
저널정보
한국토양비료학회 한국토양비료학회지 한국토양비료학회지 제54권 제3호
발행연도
2021.8
수록면
297 - 310 (14page)

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초록· 키워드

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Diaporthe citri is an important fungal pathogen which causes melanose in various citrus growing regions of the world. In our study, control of D. citri was performed by using effective biocontrol agent, Bacillus velezensis CE 100. Mycelial growth of D. citri was highly restricted by strain CE 100 in dual culture plates. Our study demonstrates large-scale inoculation of strain CE 100 in non-sterilized PB medium. In addition, bacterial culture filtrate (BCF) of strain CE 100 from the large-scale culture showed better growth inhibition of D. citri than those from small-scale culture in the laboratory. Moreover, microscopic observation revealed that hyphal morphology of the pathogen was significantly affected by BCF, probably because of various cell-wall degrading enzymes and metabolites produced by strain CE 100. In addition, 50% BCF of strain CE 100 inhibited D. citri conidial germination by over 80%. Treatments, applied by foliar spray with fungicide and strain CE 100 culture, significantly lowered melanose disease incidence more than did control. The results of this study indicate that large-scale culture of strain CE 100 was effective for control of D. citri by means of adequate active metabolites and cells. Therefore, we demonstrate biocontrol applicability of B. velezensis CE 100 on control of citrus melanose by developing a low-cost PB medium using affordable large-scale fermenter.

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ABSTRACT
Introduction
Materials and Methods
Results
Discussion
Conclusions
References

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