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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.8
- 수록면
- 451 - 457 (7page)
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Purple non-sulfur (PNS) bacterium Rhodobacter sphaeroides KD131 was studied with the aim of achieving maximum hydrogen production using various carbon and nitrogen sources at different pH conditions. Cells grew well and produced hydrogen using (NH<sub>4</sub>)<sub>2</sub>SO₄ or glutamate as a nitrogen source in combination with a carbon substrate, succinate or malate. During 48h of photo-heterotrophic fermentation under 110W/m² illumination using a halogen lamp at 30℃, 67% of 30mM succinate added was degraded and the hydrogen yield was estimated as 3.29mol H₂/mol-succinate. However, less than 30% of formate was consumed and hydrogen was not produced due to a lack of genes coding for the formate-hydrogen lyase complex of strain KD131. Initial cell concentrations of more than 0.6g dry cell weight/L-culture broth were not favorable for hydrogen evolution by cell aggregation, thus leading to substrate and light unavailability. In a modified Sistrom`s medium containing 30mM succinate with a carbon to nitrogen ratio of 12.85 (w/w), glutamate produced 1.40-fold more hydrogen compared to ammonium sulfate during the first 48h. However, ammonium sulfate was 1.78-fold more effective for extended cultivation of 96h. An initial pH range from 6.0 to 9.0 influenced cell growth and hydrogen production, and maintenance of pH 7.5 during photo- fermentation led to the increased hydrogen yield.
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참고문헌 (13)
참고문헌 신청
손한나 / 2010 / Rhodobacter sphaeroides KD131에 의한 유기산 광합성 발효 최적화 / 한국수소및신에너지학회논문집 21(2):136~142
Redwood M. D / 2009 / Integrating dark and Light biohydrogen production strategies: towards the hydrogen economy / Rev Environ Sci Biotechnol 8(2):149~185
Shi X.-Y / 2005 / Optimization of glutamate concentration and pH for H2 production from using spent media of Enterobacter cloacae DM11 / Appl Microbiol Biotechnol 68(4):533~541
SasikaLa K. / 1991 / Environmental regulation for optimal biomass yield and photoproduction of hydrogen by Rhodobacter sphaeroides O.U.001 / Int J Hydrogen Energy 16(9):597~601
Argun H. / 2009 / Effects of the substrate and cell concentration on biohydrogen production from ground wheat by combined dark and photo fermentation / Int J Hydrogen Energy 34(15):6181~6188
Redwood M. D / 2009 / Integrating dark and Light biohydrogen production strategies: towards the hydrogen economy / Rev Environ Sci Biotechnol 8(2):149~185
Shi X.-Y / 2005 / Optimization of glutamate concentration and pH for H2 production from using spent media of Enterobacter cloacae DM11 / Appl Microbiol Biotechnol 68(4):533~541
SasikaLa K. / 1991 / Environmental regulation for optimal biomass yield and photoproduction of hydrogen by Rhodobacter sphaeroides O.U.001 / Int J Hydrogen Energy 16(9):597~601
Argun H. / 2009 / Effects of the substrate and cell concentration on biohydrogen production from ground wheat by combined dark and photo fermentation / Int J Hydrogen Energy 34(15):6181~6188
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