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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.3
- 수록면
- 23 - 29 (7page)
- DOI
- 10.7841/ksbbj.2021.36.1.23
이용수
초록· 키워드
오류제보하기
Since the first outbreak in Wuhan, China, Coronavirus Disease 2019 (COVID-19) has spread rapidly around the world and there are still many confirmed cases worldwide. In this serious situation, the rapid diagnosis and classification of patients who are infected with COVID-19 are very important to individuals and society. There are two main methods of testing in COVID-19: nucleic acid testing and immunological testing, but the test currently used in the confirmatory assay is nucleic acid testing, due to its accuracy. This nucleic acid test uses RT-PCR which is using reverse transcriptase, because SARS-CoV-2 is RNA virus. One of the important factors determining accuracy in RT-PCR is the primer sequences. In other words, it is very important for the accuracy of the test that it adheres exactly to the target gene. Therefore, this review focused on primer and described the test methods for SARS-CoV-2 detection. The main targets of the primers in SARS-CoV-2 were ORF1ab/RdRp gene, E gene, N gene, and S gene. I conducted comparisons of several primer probe sets of these target genes, and also checked the primer configuration of several commercial diagnostic kits to inspect the actual utilization. As a result, most of the genes I mentioned were frequently used as targets, and most of them were highly reliable.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. CONCLUSION
REFERENCES
참고문헌 (24)
참고문헌 신청
김영진 / 2020 / COVID-19 Testing in South Korea: Current Status and the Need for Faster Diagnostics / Annals of Laboratory Medicine 40(5):349~350
Li, D. / 2020 / Primer design for quantitative real-time PCR for the emerging coronavirus SARS-CoV-2 / Theranostics 10:7150~7162
Peñarrubia, L. / 2020 / Multiple assays in a real-time RTPCR SARS-CoV-2 panel can mitigate the risk of loss of sensitivity by new genomic variants during the COVID-19 outbreak / Int J Infect Dis 97:225~229
김종식 / 2020 / SARS-CoV-2의 진단기술 / 생명과학회지 30(8):731~741
Khailany, R. A. / 2020 / Genomic characterization of a novel SARS-CoV-2 / Gene Rep 19:100682
Li, D. / 2020 / Primer design for quantitative real-time PCR for the emerging coronavirus SARS-CoV-2 / Theranostics 10:7150~7162
Peñarrubia, L. / 2020 / Multiple assays in a real-time RTPCR SARS-CoV-2 panel can mitigate the risk of loss of sensitivity by new genomic variants during the COVID-19 outbreak / Int J Infect Dis 97:225~229
김종식 / 2020 / SARS-CoV-2의 진단기술 / 생명과학회지 30(8):731~741
Khailany, R. A. / 2020 / Genomic characterization of a novel SARS-CoV-2 / Gene Rep 19:100682
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