지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.11
- 수록면
- 770 - 774 (5page)
이용수
초록· 키워드
오류제보하기
Background: Fermentation has been shown to improve the biological properties of plants and herbs. Specifically, fermentation causes decomposition and/or biotransformation of active metabolites into high-value products. Polyacetylenes are a class of polyketides with a pleiotropic profile of bioactivity.
Methods: Column chromatography was used to isolate compounds, and extensive NMR experiments were used to determine their structures. The transformation of polyacetylene in red ginseng (RG) and the production of cazaldehyde B induced by the extract of RG were identified by TLC and HPLC analyses.
Results: A new metabolite was isolated from RG fermented by Chaetomium globosum, and this new metabolite can be obtained by the biotransformation of polyacetylene in RG. Panaxytriol was found to exhibit the highest antifungal activity against C. globosum compared with other major ingredients in RG. The fungus C. globosum cultured in RG extract can metabolize panaxytriol to Metabolite A to survive, with no antifungal activity against itself. Metabolites A and B showed obvious inhibition against NO production, with ratios of 42.75±1.60 and 63.95±1.45% at 50 µM, respectively. A higher inhibitory rate on NO production was observed for Metabolite B than for a positive drug.
Conclusion: Metabolite A is a rare example of natural polyacetylene biotransformation by microbial fermentation. This biotransformation only occurred in fermented RG. The extract of RG also stimulated the production of a new natural product, cazaldehyde B, from C. globosum. The lactone in Metabolite A can decrease the cytotoxicity, which was deemed to be the intrinsic activity of polyacetylene in ginseng.
Methods: Column chromatography was used to isolate compounds, and extensive NMR experiments were used to determine their structures. The transformation of polyacetylene in red ginseng (RG) and the production of cazaldehyde B induced by the extract of RG were identified by TLC and HPLC analyses.
Results: A new metabolite was isolated from RG fermented by Chaetomium globosum, and this new metabolite can be obtained by the biotransformation of polyacetylene in RG. Panaxytriol was found to exhibit the highest antifungal activity against C. globosum compared with other major ingredients in RG. The fungus C. globosum cultured in RG extract can metabolize panaxytriol to Metabolite A to survive, with no antifungal activity against itself. Metabolites A and B showed obvious inhibition against NO production, with ratios of 42.75±1.60 and 63.95±1.45% at 50 µM, respectively. A higher inhibitory rate on NO production was observed for Metabolite B than for a positive drug.
Conclusion: Metabolite A is a rare example of natural polyacetylene biotransformation by microbial fermentation. This biotransformation only occurred in fermented RG. The extract of RG also stimulated the production of a new natural product, cazaldehyde B, from C. globosum. The lactone in Metabolite A can decrease the cytotoxicity, which was deemed to be the intrinsic activity of polyacetylene in ginseng.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusion
References
참고문헌 (17)
참고문헌 신청
임경택 / 2016 / Ginseng, the natural effectual antiviral: Protective effects of Korean Red Ginseng against viral infection / Journal of Ginseng Research 40(4):309~314
이상명 / 2015 / Characterization of Korean Red Ginseng (Panax ginseng Meyer): History, preparation method, and chemical composition / Journal of Ginseng Research 39(4):384~391
Hussain A / 2016 / Fermentation, a feasible strategy for enhancing bioactivity of herbal medicines / Food Res Inter 81:1~16
이승홍 / 2015 / Antioxidant and Anti-inflammatory Activities of the Ethanolic Extract of Fermented Red Ginseng Marc / Food Science and Biotechnology 24(2):651~657
Li Z / 2016 / Korean ginseng berry fermented by mycotoxin non-producing Aspergillus Niger and Aspergillus oryzae : ginsenoside analyses and anti-proliferative activities / Biol Pharm Bull 39:1461~1467
이상명 / 2015 / Characterization of Korean Red Ginseng (Panax ginseng Meyer): History, preparation method, and chemical composition / Journal of Ginseng Research 39(4):384~391
Hussain A / 2016 / Fermentation, a feasible strategy for enhancing bioactivity of herbal medicines / Food Res Inter 81:1~16
이승홍 / 2015 / Antioxidant and Anti-inflammatory Activities of the Ethanolic Extract of Fermented Red Ginseng Marc / Food Science and Biotechnology 24(2):651~657
Li Z / 2016 / Korean ginseng berry fermented by mycotoxin non-producing Aspergillus Niger and Aspergillus oryzae : ginsenoside analyses and anti-proliferative activities / Biol Pharm Bull 39:1461~1467
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