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논문 기본 정보

자료유형
학술저널
저자정보
Lim, Jin-Ho (College of Oriental Medicine, Daegu Haany University) Lee, Jong-Rok (College of Oriental Medicine, Daegu Haany University) Kim, Sang-Chan (College of Oriental Medicine, Daegu Haany University, Development Team for The New Drug of Oriental Medicine [BK21 program] Daegu Haany University) Jee, Seon-Young (College of Oriental Medicine, Daegu Haany University)
저널정보
경희한의학연구센터 Oriental pharmacy and experimental medicine Oriental pharmacy and experimental medicine 제7권 제3호
발행연도
2007.1
수록면
321 - 329 (9page)

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The present study was conducted to evaluate the effect of Yongdamsagantang (YST) on the regulatory mechanism of cytokines and nitric oxide (NO) for the immunological activities in RAW 264.7 cells. After the treatment of YST water extract, cell viability was measured by MTT assay, and NO production was monitored by measuring the nitrite content in culture medium. Inducible nitric oxide synthase (iNOS) and phospholylation of inhibitor of nuclear factor kappa B alpha ($p-I{\kappa}B{\alpha}$) were determined by Immunoblot analysis, and levels of cytokine were analyzed by sandwich immunoassays. Results provided evidences that YST inhibited the production of NO. iNOS, and interleukin-6, and the activation of $p-I{\kappa}B{\alpha}$ in RAW 264.7 cells activated with lipopolysaccharide. These findings showed that YST could have some anti-inflammatory effects which might play a role in therapy in Gram-negative bacterial infections.

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