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논문 기본 정보

자료유형
학술저널
저자정보
Madanagopal, Nalini (Department of Bioresource Sciences, Andong National University) Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University)
저널정보
한국응용곤충학회 Journal of Asia-Pacific Entomology Journal of asia-pacific entomology 제10권 제3호
발행연도
2007.1
수록면
229 - 237 (9page)

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Lectins due to their affinity to carbohydrate moiety are involved in diverse functions like cell attachment in embryogenesis, organogenesis and cellular trafficking as well as nonself recognition in immune responses. Agglutinating activity was detectable in Plutella xylostella (Yponomeutidae: Lepidoptera) against 14 different species including bacterial and yeast cells, among which the whole body homogenate of P. xylostella agglutinated Providencia vermicola, Flavobacterium sp., and Saccharomyces cerevisiae with high titers. On analysis of physico-chemical properties, this putative agglutinating factor (s) was specifically dependent on the presence of $Ca^{++}$ for its activity and was reversibly sensitive to EDTA. The agglutinating activity was stable at pH 6-8, but was heat-labile. The agglutinating factor (s) was proteinaceous in nature as it was completely precipitable by ammonium sulphate. Its carbohydrate binding activity was demonstrated by inhibition assay, which revealed that methyl ${\alpha}$-D-mannopyranoside inhibited agglutination against P. vermicola. In contrast, P. xylostella parasitized by an endoparasitoid wasp, Cotesia plutellae (Braconidae: Hymenoptera), also showed the agglutination properties with somewhat higher activity than the nonparasitized. Carbohydrate inhibition assay with methyl ${\alpha}$-D-mannopyranoside was detectable at one-fold higher concentration in the homogenate of the parasitized larvae, suggesting that the agglutinating factor (s) is inducible or due to de novo parasitism-specific synthesis. These results suggest the presence of calcium-dependent lectin in P. xylostella and an alteration in the agglutinating property by C. plutellae parasitization.

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