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자료유형
학술저널
저자정보
Lee, Kee-Woo (Department of Bioelectronic Engineering, Andong National University) Cho, Sung-Hwan (Department of Agricultural Biology, Andong National Biology) Lee, Hyuk-Soo (Department of Bioelectronic Engineering, Andong National University) Choi, Jae-Young (School of Agricultural Biotechnology, Seoul National University) Je, Yeon-Ho (School of Agricultural Biotechnology, Seoul National University) Kim, Yong-Gyun (Department of Agricultural Biology, Andong National Biology)
저널정보
한국응용곤충학회 Journal of Asia-Pacific Entomology Journal of asia-pacific entomology 제8권 제3호
발행연도
2005.1
수록면
249 - 255 (7page)

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A genome project has been launched and aims to sequence total genome of Cotesia plutellae bracovirus (CpBV). This on-going research has identified several open reading frames (ORFs) including an EP1-like protein (ELP1). This study was intended to analyze gene expression of CpBV-ELP1 in the parasitized diamondback moth, Plutella xylostella. CpBV-ELP genomic DNA contains one intron (778 bp long). Its ORF consists of 726 bp encoding 241 amino acid residues. The hypothetical CpBV-ELP1 protein is predicted as 27,787.83 Da and possesses N-terminal signal peptide plus three potential N-glycosylation sites. Its amino acid sequence exhibits high homology with EP1 genes from C. congregata or C. karyai bracovirus. A reverse transcriptase-polymerase chain reaction (RT-PCR) indicated that CpBV-ELP1 was expressed only in P. xylostella parasitized by C. plutellae. The expression levels were measured by real time quantitative RT-PCRs during entire parasitization period at $25^{\circ}C$ culturing temperature. The expression began at the first day of parasitization and increased with the parasitization period. The ORF PCR product was cloned, over-expressed, and molecular weight of the purified protein was about 30 kDa.

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