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자료유형
학술저널
저자정보
Lee, Min-Ah (Department of Molecular Biology, Dong-eui University) Chun, Hyun-Sik (Department of Crop Science & Biotechnology, Jinju National University) Kim, Jin-Woo (Department of Crop Science & Biotechnology, Jinju National University) Lee, Hyub (Department of Crop Science & Biotechnology, Jinju National University) Lee, Dong-Hee (Genomine Inc.) Lee, Chin-Bum (Department of Molecular Biology, Dong-eui University)
저널정보
한국식물학회 식물학회지 식물학회지 제47권 제2호
발행연도
2004.1
수록면
117 - 123 (7page)

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We studied changes in biochemical and physiological status, level of oxidative damage, and antioxidant enzyme activities in detached leaves of cucumber plants (Cucumis sativus L. cv. Pyunggangnaebyungsamchuk) that were exposed to a low temperature (4$^{\circ}C$). Chlorophyll fluorescence ( $F_{v/}$ $F_{m}$) declined during the chilling treatment, but was slowly restored after the tissues were returned to $25^{\circ}C$. Likewise, the fluorescence quenching coefficient and relative water content decreased during the stress period, but then increased during recovery. In contrast, we detected a significant rise in protein and hydrogen peroxide contents in the chilled leaves, as well as higher activities for superoxide dismutase, ascorbate peroxidase, peroxidase, and glutathione reductase. However, the level of catalase decreased not only during chilling but also after 24 h of recovery. These results indicate that exposure to low temperatures acts as an oxidative stress. Moreover, we propose that a regulating mechanism exists in the detached cucumber leaves and contains an antioxidant defense system that induces active oxygen species, thereby alleviating the effects of chilling stress within 12 h.h.h.

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