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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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An effective and reproducible protocol for the micropropagation of Kigelia pinnata L. through high frequency callus regeneration is described. Seeds were surface sterilized before culturing on Gamborgs basal medium (85 medium). After two weeks the cotyledonary node along with a portion of the hypocotyl were carefully excised from well-developed embryos and subcultured on 85 medium supplemented with different concentrations of 2,4-D and BAP. The cultured cotyledonary node explants showed callus formation at the base of the lower cut end of the hypocotyl. This callus showed shoot initiation after two weeks of subculture on the regeneration medium supplemented with various concentrations of BAP alone or in combination with NAA. The highest number of shoot regeneration occurred on medium containing 5 $\mu$M BAP and 0.1 $\mu$M NAA. The optimum rooting of the regenerated shoots was observed on $\frac{1}{2}$B5 medium supplemented with 4 $\mu$M IBA. Micropropagated plants were successfully established in soil in field condition with a survival frequency of 100%.
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