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논문 기본 정보

자료유형
학술저널
저자정보
Kwon, Dae-Jin (School of Veterinary Medicine, Kangwon National University) Park, Joo-Hee (School of Veterinary Medicine, Kangwon National University) Hwang, Hwan-Sub (Gangwon Livestock Research Center) Park, Yeon-Soo (Gangwon Livestock Research Center) Park, Choon-Keun (College of Animal Life Science, Kangwon National University) Yang, Boo-Keun (College of Animal Life Science, Kangwon National University) Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제31권 제4호
발행연도
2007.1
수록면
235 - 240 (6page)

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초록· 키워드

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This study was conducted to examine the viability of Korean native striped cattle (Bos namadicus Falconer, Chikso) clone embryos after embryo transfer. Chikso somatic cell nuclear transfer (SCNT) embryos were produced by fusion of ear skin cells derived from a female Chikso with enucleated oocytes matured in vitro for 18-24 hr. After in vitro culture of SCNT embryos for 7 to 8 days, fresh or vitrified blastocysts derived from SCNT were transferred into a uterine horn of recipient cows. Fifteen of total 43 recipients were pregnant at Day 50 and 4 recipients were maintained to term. Three IVF-derived calves and 1 clone Chikso calf were born. Pregnancy rate was higher when fresh embryos were transferred to recipients compared to vitrified embryos, but development to term was not different between both groups. The clone Chikso calf died at 5 days after birth due to the fullness of amniotic fluid in rumen and the infection of umbilical cord. The result of the present study shows that clone Chikso calf can produced from the embryo transfer of SCNT embryos, however, solution of abortion problem is necessary to improve the cloning efficiency.

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