Derivation of Neural Precursor Cells from Human Embryonic Stem Cells

Kim Sehee; Hong Ji Young; Joo So Yeon; Kim Jae Hwan; Moon Shin Yong; Yoon Hyun Soo; Kim Doo Han; Chung Hyung Min; Choi Seong-Jun

추천

검색

자료유형: 학술저널

저자정보: Kim Sehee (Cell and Gene Therapy Research Institute, Pochon CHA University College of Medicine) Hong Ji Young (Cell and Gene Therapy Research Institute, Pochon CHA University College of Medicine) Joo So Yeon (Cell and Gene Therapy Research Institute, Pochon CHA University College of Medicine) Kim Jae Hwan (Cell and Gene Therapy Research Institute, Pochon CHA University College of Medicine) Moon Shin Yong (Cell and Gene Therapy Research Institute, Pochon CHA University College of Medicine) Yoon Hyun Soo (Cell and Gene Therapy Research Institute, Pochon CHA University College of Medicine) Kim Doo Han (Cell and Gene Therapy Research Institute, Pochon CHA University College of Medicine) Chung Hyung Min (Cell and Gene Therapy Research Institute, Pochon CHA University College of Medicine) Choi Seong-Jun (Cell and Gene Therapy Research Institute, Pochon CHA University College of Medicine)

저널정보: 한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제28권 제4호

발행연도: 2004.1

수록면: 247 - 252 (6page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Human embryonic stem (ES) cells are derived from the inner cell mass of the preimplantation embryo. Human ES cells have the capacity to differentiate into various types of cells in the body. Human ES cells are indefinite source of cells for cell therapy in various degenerative disorders including neuronal disorders. Directed differentiation of human ES cells is a prerequisite for their clinical application. The objective of this study is to develop the culture condition for the derivation of neural precursor cells from human ES cells. Neural precursor cells were derived from human ES cells in a stepwise culture condition. Neural precursor cells in the form of neural rosette structures developed into neurospheres when cultured in suspension. Suspension culture of neurospheres has been maintained over 4 months. Expressions of nestin, soxl, sox2, pax3 and pax6 transcripts were upregulated during differentiation into neural precursor cells by RT-PCR analysis. In contrast, expression of oct4 was dramatically downregulated in neural precursor cells. Immunocytochemical analyses of neural precursor cells demonstrated expression of nestin and SOX1. When induced to differentiate on an adhesive substrate, neuro-spheres were able to differentiate into three lineages of neural systems, including neurons, astrocytes and oligo-dendrocytes. Transcripts of sox1 and pax6 were downregulated during differentiation of neural precursor cells into neurons. In contrast, expression of map2ab was elevated in the differentiated cells, relative to those in neural precursor cells. Neurons derived from neural precursor cells expressed NCAM, Tuj1, MAP2ab, NeuN and NF200 in immunocytochemical analyses. Presence of astrocytes was confirmed by expression of GFAP immuno-cytochemically. Oligodendrocytes were also observed by positive immuno-reactivities against oligodendrocyte marker O1. Results of this study demonstrate that a stepwise culture condition is developed for the derivation of neural precursor cells from human ES cells.

#Embryonic stem cell #Differentiation #Neural precursor cell #Human

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!