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자료유형
학술저널
저자정보
Jin, Dong-Chung (School of Bioscience and Biotechnology, Genome Research Center, Chungnam National University) Kim, Joong-Soon (School of Bioscience and Biotechnology, Genome Research Center, Chungnam National University) Park, ji-Young (School of Bioscience and Biotechnology, Genome Research Center, Chungnam National University) Bong, Jae-Wook (School of Bioscience and Biotechnology, Genome Research Center, Chungnam National University) Hur, Yoon-Kang (School of Bioscience and Biotechnology, Genome Research Center, Chungnam National University)
저널정보
한국광과학회 Journal of photoscience : an international journal officail organ of the korean society of photoscience Journal of photoscience : an international journal officail organ of the korean society of photoscience 제12권 제2호
발행연도
2005.1
수록면
75 - 82 (8page)

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Rumex acetosa L. is a dioecious flowering plant with well developed sex chromosome system: 2n = 12 + XX in the female plants and 2n = 12 + XY1Y2 in the male plants. To isolate sex-linked DNA, we carried out chromosome micromanipulation, followed by DOP-PCR, AFLP of the PCR products, reverse Southern hybridization and sequence analysis. From 500 AFLP specific clones, 13 X-chromosome and 5 Y-chromosome specific clones were obtained. Except one clone RADAX-239 ($\underline{R}umex\;\underline{a}-\underline{D}OP-PCR-\underline{A}FLP-\underline{Y}-chromosome\;specific$), all clones appear to be R. acetosa plant-specific sequences and non-coding sequences. Southern blot analysis using these clones could not discriminate genomic DNAs either from male or female plants. Results of this study imply that both autosome-origin and degeneration of sex chromosomes are prevalent in plant systems.

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