메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
Kwak, Seong-Song (Laboratory of Veterinary Embryology and Biotechnology [VETEMBIO], College of Veterinary Medicine, Chungbuk National University) Jeong, Se-Heon (Laboratory of Veterinary Embryology and Biotechnology [VETEMBIO], College of Veterinary Medicine, Chungbuk National University) Jang, Seung-Hoon (Laboratory of Veterinary Embryology and Biotechnology [VETEMBIO], College of Veterinary Medicine, Chungbuk National University) Jeon, Yu-Byeol (Laboratory of Veterinary Embryology and Biotechnology [VETEMBIO], College of Veterinary Medicine, Chungbuk National University) Nam, Young-Hee (Laboratory of Veterinary Embryology and Biotechnology [VETEMBIO], College of Veterinary Medicine, Chungbuk National University) Biswas, Dibyendu (Laboratory of Veterinary Embryology and Biotechnology [VETEMBIO], College of Veterinary Medicine, Chungbuk National University) Lee, Wan-Kyu (Laboratory of Veterinary Bacteriology, College of Veterinary Medicine, Chungbuk National University) Hyun, Sang-Hwan (Laboratory of Veterinary Embryology and Biotechnology [VETEMBIO], College of Veterinary Medicine, Chungbuk National Universi)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제25권 제4호
발행연도
2010.1
수록면
267 - 272 (6page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
The present study was to investigate the source of contamination during semen processing for in vitro uses. In the present study, frozen semen was prepared from liquid semen in our laboratory for in vitro fertilization (IVF) experiments due to lack of fresh semen. Antibiotics were added in the frozen semen extender (kanamycin and gentamicin) and in vitro culture (IVC) medium (gentamicin) for further inhibiting growth of microorganisms. Nevertheless, proliferations of microorganisms were observed in IVC culture drop during culturing of IVF embryos using frozen semen. Randomly 3 samples were taken from the liquid semen, frozen semen and egg yolk. Contaminated IVC medium, frozen-thawed semen, liquid semen and egg yolk were cultured in de Man, Rogosa and Sharpe (MRS) agar medium. Whitish colonies were detected in contaminated IVC drop, frozen-thawed semen samples and egg yolk but no colonies were formed in liquid semen samples. Gram-negative and rod-shaped identical bacteria were found in both frozen-thawed semen sample and contaminated IVC drop and egg yolk samples. Enterobacter cloacae were confirmed by API 20E kit according to manufacturer's instruction with identification value (% ID) 94.3% and T index 0.88. Antibiotic susceptibility tests were done according to Clinical and Laboratory Standards Institute (CLSI) by using ampicillin, amikacin, cephalothin, gentamicin, kanamycin, tetracycline, oxytetracycline, sulfamethoxazole trimethoprim, norfloxacin and ciprofloxacin test. Among them Enterobacter cloacae were resistant to ampicillin, amikacin, cephalothin, gentamicin, kanamycin but susceptible to tetracycline, oxytetracycline, sulfamethoxazole trimethoprim, norfloxacin and ciprofloxacin. From these findings it could be suggested that this contamination sources might be from egg yolk.

목차

등록된 정보가 없습니다.

참고문헌 (0)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0