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논문 기본 정보

자료유형
학술저널
저자정보
Park, Bola (Labortory of Theriogenology, College of Veterinary Medicine, Kangwon National University) Lee, Joohyeong (Labortory of Theriogenology, College of Veterinary Medicine, Kangwon National University) Lee, Yongjin (Labortory of Theriogenology, College of Veterinary Medicine, Kangwon National University) Elahi, Fazle (Labortory of Theriogenology, College of Veterinary Medicine, Kangwon National University) Jeon, Yubyeol (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) Hyun, Sang-Hwan (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) Lee, Eunsong (Labortory of Theriogenology, College of Veterinary Medicine, Kangwon National University)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제28권 제2호
발행연도
2013.1
수록면
133 - 139 (7page)

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초록· 키워드

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The objective of this study was to determine the effect of post-activation treatment with cytoskeletal regulators in combination with or without 6-dimethylaminopurine (DMAP) on embryonic development of pig oocytes after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT). PA and SCNT oocytes were produced by using in vitro-matured pig oocytes and treated for 4 h after electric activation with $0.5{\mu}M$ latrunculin A (LA), $10.4{\mu}M$ cytochalasins B (CB), and $4.9{\mu}M$ cytochalasins D (CD) together with none or 2 mM DMAP. Post-activation treatment of PA oocytes with LA, CB, and CD did not alter embryo cleavage (85.8~88.6%), blastocyst formation (30.7~ 32.4%), and mean cell number of blastocysts (33.5~33.8 cells/blastocyst). When PA oocytes were treated with LA, CB, and CD in combination with DMAP, blastocyst formation was significantly (P<0.05) improved by CB+DMAP (42.5%) compared to LA+DMAP (28.0%) and CD+DMAP (25.1%), but no significant differences were found in embryo cleavage (77.5~78.0%) and mean blastocyst cell number (33.6~35.0 cells) among the three groups. In SCNT, blastocyst formation was significantly (P<0.05) increased by post-activation treatment with LA+DMAP (32.9%) and CD+DMAP (35.0%) compared to CB+DMAP (22.0%) while embryo cleavage (85.5~85.7%) and blastocyst cell number (41.1~43.8 cells) were not influenced. All three treatments (LA, CB, and CD with DMAP) effectively inhibited pseudo-polar body extrusion in SCNT oocytes. The proportions of oocytes showing single pronucleus formation were 89.6%, 83.9%, and 93.3%, respectively with the increased tendency (P<0.1) by LA+DMAP and CD+ DMAP compared to CB+DMAP. Our results demonstrate that post-activation treatment with LA or CD in combination with DMAP improves pre-implantation development of SCNT embryos and the stimulating effect of cytoskeletal modifiers on embryonic development is differentially shown depending on the origin (PA or SCNT) of embryos in pigs.

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