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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Hanna (Division of Applied Animal Science, College of Animal Life Science, Kangwon National University) Lee, Yongjin (College of Veterinary Medicine, Kangwon National University) Park, Bola (College of Veterinary Medicine, Kangwon National University) Elahi, Fazle (College of Veterinary Medicine, Kangwon National University) Lee, Joohyeong (College of Veterinary Medicine, Kangwon National University) Choi, Jung Hoon (College of Veterinary Medicine, Kangwon National University) Lee, Seung Tae (Division of Applied Animal Science, College of Animal Life Science, Kangwon National University) Park, Choon-Keun (Division of Applied Animal Science, College of Animal Life Science, Kangwon National University) Hyun, Sang-Hwan (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) Lee, Eunsong (College of Veterinary Medicine, Kangwon National University)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제29권 제4호
발행연도
2014.1
수록면
361 - 368 (8page)

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초록· 키워드

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This study was designed to evaluate the effect of bovine serum albumin (BSA) in a maturation medium on oocyte maturation and embryonic development in pigs. Immature pig oocytes were matured for 44 h in a medium supplemented with 0.4% (w/v) BSA, 0.1% (w/v) polyvinyl alcohol (PVA), or 10% (v/v) pig follicular fluid (PFF). After IVM, oocytes reached metaphase II stage were activated for parthenogenesis (PA) or used as cytoplasts for somatic cell nuclear transfer (SCNT). Nuclear maturation (89.5%, 90.7% and 91.3% for BSA, PVA and PFF, respectively) and intraoocyte glutathione contents (1.20, 1.16 and 1.00 pixels/oocyte for BSA, PVA and PFF, respectively) were not altered by the macromolecules added to maturation medium. IVM of oocytes in a medium containing BSA (21.4%) and PVA (20.7%) showed significantly lower blastocyst formation after PA than culture in medium with PFF (39.2%). After SCNT, oocytes matured in medium with BSA showed decreased embryonic development to the blastocyst stage (9.2%) compared to those matured in medium with PFF (28.9%), while 23.6% of SCNT oocytes matured in medium with PVA developed to the blastocyst stage. When the effect of BSA in a maturation medium during the first 22 h and the second 22 h of IVM in combination with PFF or PVA was examined, PVA-BSA showed a higher nuclear maturation (94.1%) than BSA-PFF (84.5%). However, there was no significant difference in the blastocyst formation among tested combinations (47.3, 52.2, 50.0, 44.4 and 49.0% for PFF-PFF, PFF-BSA, PVA-BSA, BSA-PVA and BSA-PFF, respectively). Our results demonstrate that BSA and PVA added to maturation medium can support oocyte maturation comparable to PFF-supplemented medium. However, maturation of oocytes in a BSA-containing medium decreases embryonic development after PA and SCNT when compared with the medium supplemented with PFF.

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