지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 799 - 807 (9page)
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초록· 키워드
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Meat source fraud and adulteration scandals have led to consumer demands for accurate meat identification methods. Nucleotide amplification assays have been proposed as an alternative method to protein-based assays for meat identification. In this study, we designed Loop-mediated isothermal amplification (LAMP) assays targeting species-specific mitochondrial DNA to identify and discriminate eight meat species; cattle, pig, horse, goat, sheep, chicken, duck, and turkey. The LAMP primer sets were designed and the target genes were discriminated according to their unique annealing temperature generated by annealing curve analysis. Their unique annealing temperatures were found to be $85.56{\pm}0.07^{\circ}C$ for cattle, $84.96{\pm}0.08^{\circ}C$ for pig, and $85.99{\pm}0.05^{\circ}C$ for horse in the BSE-LAMP set (Bos taurus, Sus scrofa domesticus and Equus caballus); $84.91{\pm}0.11^{\circ}C$ for goat and $83.90{\pm}0.11^{\circ}C$ for sheep in the CO-LAMP set (Capra hircus and Ovis aries); and $86.31{\pm}0.23^{\circ}C$ for chicken, $88.66{\pm}0.12^{\circ}C$ for duck, and $84.49{\pm}0.08^{\circ}C$ for turkey in the GAM-LAMP set (Gallus gallus, Anas platyrhynchos and Meleagris gallopavo). No cross-reactivity was observed in each set. The limits of detection (LODs) of the LAMP assays in raw and cooked meat were determined from $10pg/{\mu}L$ to $100fg/{\mu}L$ levels, and LODs in raw and cooked meat admixtures were determined from 0.01% to 0.0001% levels. The assays were performed within 30 min and showed greater sensitivity than that of the PCR assays. These novel LAMP assays provide a simple, rapid, accurate, and sensitive technology for discrimination of eight meat species.
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참고문헌 (35)
참고문헌 신청
Ahmed, M. U / 2010 / Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensor / Food Control 21:599~605
Avise, J. C / 1987 / Intraspecific phylogeography-the mitochondrial-DNA bridge between population-genetics and systematics / Annu. Rev. Ecol. Syst 18:489~522
Ballin, N. Z / 2009 / Species determination-Can we detect and quantify meat adulteration? / Meat Sci 83:165~174
Bottero, M. T / 2011 / Animal species identification in food products: evolution of biomolecular methods / Vet. J 190:34~38
Yeong-Hsiang Cheng / 2006 / Investigation of Goats' Milk Adulteration with Cows' Milk by PCR / ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES 19(10):1503~1507
Avise, J. C / 1987 / Intraspecific phylogeography-the mitochondrial-DNA bridge between population-genetics and systematics / Annu. Rev. Ecol. Syst 18:489~522
Ballin, N. Z / 2009 / Species determination-Can we detect and quantify meat adulteration? / Meat Sci 83:165~174
Bottero, M. T / 2011 / Animal species identification in food products: evolution of biomolecular methods / Vet. J 190:34~38
Yeong-Hsiang Cheng / 2006 / Investigation of Goats' Milk Adulteration with Cows' Milk by PCR / ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES 19(10):1503~1507
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