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자료유형
학술저널
저자정보
Kim, Hack-Youn (Department of Food and Nutrition, Sookmyung Women's University) Kim, Cheon-Jei (Department of Animal Resources Science, Kongju National University) Han, Sung Gu (Department of Animal Resources Science, Kongju National University) Lee, Sunah (Department of Food and Nutrition, Sookmyung Women's University) Choi, Kyoung-Hee (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) Yoon, Yohan (Department of Food and Nutrition, Sookmyung Women's University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제34권 제1호
발행연도
2014.1
수록면
20 - 25 (6page)

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This study evaluated the effects of frankfurter fat content on Listeria monocytogenes resistance to heat stress and gastric fluid, and the Caco-2 cell invasion efficiency of the pathogen. A 10-strain mixture of L. monocytogenes was inoculated on frankfurters formulated with 10%, 20%, and 30% fat content (10%: F10, 20%: F20, 30%: F30) and stored at $10^{\circ}C$ for 30 d. The samples were analyzed for L. monocytogenes resistance to heat stress and a simulated gastric fluid challenge. The total bacteria and L. monocytogenes survival rates were measured on tryptic soy agar plus 0.6% yeast extract and Palcam agar, respectively. L. monocytogenes colonies inoculated on F10, F20, and F30 samples were used for a Caco-2 cell invasion assay. In general, no obvious differences were observed between the survival rates of total bacteria and L. monocytogenes grown on different fat contents under heat stress and gastric fluid challenge. However, L. monocytogenes obtained from the F30 samples had a significantly higher Caco-2 cell invasion efficiency than those in the F10 and F20 samples (p<0.05). These results indicate that although high fat content in food may not be related to L. monocytogenes resistance to heat stress and gastric fluid, it may increase the Caco-2 cell invasion efficiency of the pathogen.

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