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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 701 - 709 (9page)
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초록· 키워드
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This study was performed to establish an effective extraction method of pig placenta extract that could be used for a putative functional food supplement with immunomodulatory effects. In the present study, we used different temperatures (4, 37, 60, 80, and $100^{\circ}C$) and different solvents (chloroform, NaOH, and phosphate buffered saline [PBS]) to extract the pig placenta. Among the different placenta extracts yielded by the different extraction methods, placenta extract (PE) in PBS at $80^{\circ}C$ for 30 min (referred to as PE-PBS80) showed a significant increase of nitric oxide production of up to 22.97 ${\mu}M/10^5$ cells at a 1 mg/mL dose (p<0.05 ) in J774A.1 cells than other extracts and control tested. Using PE-PBS80, further animal challenges were performed to identify the immune-enhanced effects. As a result, orally administered PE-PBS80 showed a significant increase in blood T and B cell activities and immunoglobulin (IgG and IgM) production. IgG and IgM levels increased to 41.53 mg/mL at a 20 mg dose on day 7 and to 27.38 mg/mL at a 10 mg dose on day 14, respectively (p<0.05). Furthermore, PE-PBS80 was also able to significantly enhance the immune modulator cytokine levels (p<0.05) compared to the control and vehicle treatments. Among the evaluated cytokines, the tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) level increased to 28.89 pg/mL at extract doses of 20 and 50 mg, the interleukin-$1{\beta}$ (IL-$1{\beta}$) level increased to 21.52 pg/mL at extract doses of 10, 20, 50 and 75 mg and the interferon (IFN)-${\gamma}$ level increased to 18.24 pg/mL at extract doses of 10, 20, and 50 mg. Therefore, this study presents an effective method for extracting pig placenta extracts and also demonstrates that pig placenta extracts had significant immunomodulatory effects not only at the cellular level but also in a mouse model, suggesting that this material could be used as an excellent candidate functional food supplement.
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참고문헌 (30)
참고문헌 신청
Banerjee, K. K. / 1992 / Anti-inammatory effect of human placental extract: a biochemicalmechanistic approach / Rivista. Europea. per le ScienzeMedichee. Farmacologiche 14:361~366
Benedict, C. / 2009 / Enhancing influence of intranasal interleukin-6 on slow-wave activity and memory consolidationduring sleep / FASEB J 23:3629~3636
Biswas, T. K. / 2001 / Wound healing activity ofhuman placental extracts in rats / Act. Pharmacol. Sin 22:1113~1116
Bobe, P. / 1984 / Modulation of mouse anti-SRBC antibody response by placentalextracts / Cell. Immunol 89:355~364
Bobe, P. / 1986 / Modulation of mouse anti-SRBC antibodyresponses by placental extracts. 11. Antigen specificity andregulatory role of B and T cell populations affected by twodistinct placental fractions / J. Immunol 136:574~581
Benedict, C. / 2009 / Enhancing influence of intranasal interleukin-6 on slow-wave activity and memory consolidationduring sleep / FASEB J 23:3629~3636
Biswas, T. K. / 2001 / Wound healing activity ofhuman placental extracts in rats / Act. Pharmacol. Sin 22:1113~1116
Bobe, P. / 1984 / Modulation of mouse anti-SRBC antibody response by placentalextracts / Cell. Immunol 89:355~364
Bobe, P. / 1986 / Modulation of mouse anti-SRBC antibodyresponses by placental extracts. 11. Antigen specificity andregulatory role of B and T cell populations affected by twodistinct placental fractions / J. Immunol 136:574~581
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