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논문 기본 정보

자료유형
학술저널
저자정보
Han, Sun-Kyung (College of Veterinary Medicine, Chungbuk National University) Shin, Myeong-Su (College of Veterinary Medicine, Chungbuk National University) Park, Ho-Eun (College of Veterinary Medicine, Chungbuk National University) Kim, So-Young (Functional Food & Nutrition Division, Department of Agrofood Resource, National Academy of Agricultural Science, Rural Development Administration) Lee, Wan-Kyu (College of Veterinary Medicine, Chungbuk National University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제34권 제5호
발행연도
2014.1
수록면
614 - 621 (8page)

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초록· 키워드

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This study was conducted to isolate and characterize bacteriocin-producing bacteria against Clostridium perfringens (C. perfringens) from domestic animals to determine their usefulness as probiotics. Bacteriocin-producing bacteria were isolated from pig feces by the spot-on-lawn method. A total of 1,370 bacterial stains were isolated, and six were tentatively selected after identifying the inhibitory activity against the pathogenic indicator C. perfringens KCTC 3269 and KCTC 5100. The selected strains were identified as Enterococcus faecalis (E. faecalis) by 16s rRNA sequencing. Most of the isolated bacterial strains were resistant to 0.5% bile salts for 48 h and remained viable after 2 h at pH 3.0. Some E. faecalis also showed strong inhibitory activity against Listeria monocytogenes KCTC 3569, KCTC 3586 and KCTC 3710. In the present study, we finally selected E. faecalis AP 216 and AP 45 strain based on probiotic selection criteria such as antimicrobial activity against C. perfringens and tolerance to acid and bile salts. The bacteriocins of E. faecalis AP 216 and AP 45 strains were highly thermostable, showing anticlostridial activities even after incubation at $121^{\circ}C$ for 15 min. These bacteriocin-producing bacteria and/or bacteriocins could be used in feed manufacturing as probiotics as an alternative to antibiotics in the livestock industry.

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