Preparation and Characterization of Antioxidant Peptides from Fermented Goat Placenta

Hou, Yinchen; Zhou, Jiejing; Liu, Wangwang; Cheng, Yongxia; Wu, Li; Yang, Gongming

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자료유형: 학술저널

저자정보: Hou, Yinchen (College of Food Science, South China Agricultural University) Zhou, Jiejing (College of Food Science, South China Agricultural University) Liu, Wangwang (College of Food Science, South China Agricultural University) Cheng, Yongxia (College of Food Science, South China Agricultural University) Wu, Li (College of Food Science, South China Agricultural University) Yang, Gongming (College of Food Science, South China Agricultural University)

저널정보: 한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제34권 제6호

발행연도: 2014.1

수록면: 769 - 776 (8page)

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The goat placenta was fermented by Bacillus subtilis and the optimal fermentation parameters of strongest antioxidant capacity of peptides were obtained using response surface methodology (RSM). The effects of fermentation time, initial pH value and glucose content on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity of the goat peptides were well fitted to a quadric equation with high determination coefficients. According to the data analysis of design expert, the strongest DPPH radical scavenging capacity value was obtained with the following conditions: content of glucose was 2.23%, initial pH value was 7.00 and fermentation time was 32.15 h. The DPPH radical scavenging capacity commonly referring antioxidant activity showed a concentration dependency and increased with increasing peptide concentration. The effects of temperature and pH were assessed to determine the stability of antioxidant peptides prepared from goat placenta. Antioxidant peptides showed good stabilities when temperature was lower than $70^{\circ}C$. However, the antioxidant peptides lost antioxidant activities rapidly under alkaline and excessive acid condition. Ultrafiltration technique was performed to separate fermentation broth with different Mw (molecular weight). It was found that peptides in the range of < 3 KDa mainly accounted for the antioxidant activities.

#Bacillus subtilis #goat placenta #antioxidant #peptide #fermentation

참고문헌 (35)

참고문헌 신청

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